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首页> 外文期刊>Crystal growth & design >Effects of forced solution flow on protein-crystal quality and growth process
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Effects of forced solution flow on protein-crystal quality and growth process

机译：强制溶液流动对蛋白质晶体质量和生长过程的影响

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X-ray diffraction experiments were performed to study the effect of forced solution flow on protein-crystal quality. The tetragonal crystals of hen egg white lysozyme were grown under flow in the rate range of 0 ( quiescent condition) to 1000 mu m/s. The quality of the crystals grown under flow was superior to that of crystals grown under quiescent conditions. To investigate the mechanism of the quality improvement, we observed the surface morphology of the ( 110) face using atomic force microscopy (AFM). Under quiescent conditions, the two-dimensional (2D) nuclei were randomly formed over the surface. Under solution flow, a hillock consisting of 2D islands was formed around the facet center. The step density under flow was higher than that under quiescent conditions. The step growth rate decreased with increasing flow rate because of the step-density increase. We found that there was a strong correlation between the crystal quality and the step growth rate. The results suggested that the slow growth decreases the molecular misorientation at the kink site, resulting in an improvement in crystal quality.
机译：进行了X射线衍射实验，以研究强制溶液流动对蛋白质晶体质量的影响。鸡蛋清溶菌酶的四方晶体在0（静止条件）至1000μm / s的流速范围内流动。在流动条件下生长的晶体的质量优于在静态条件下生长的晶体的质量。为了研究质量改善的机制，我们使用原子力显微镜（AFM）观察了（110）面的表面形态。在静态条件下，二维（2D）原子核在表面上随机形成。在溶液流作用下，围绕小平面中心形成了由2D岛组成的小丘。流动条件下的台阶密度高于静止条件下的台阶密度。步长增长率随着流速的增加而降低，因为步长密度增加。我们发现晶体质量与台阶生长速率之间存在很强的相关性。结果表明，缓慢的生长减少了扭结部位的分子取向错误，从而改善了晶体质量。

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