...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>ACS Synthetic Biology >Mutagenesis of the Microcystin Tailoring and Transport Proteins in a Heterologous Cyanotoxin Expression System
【24h】

Mutagenesis of the Microcystin Tailoring and Transport Proteins in a Heterologous Cyanotoxin Expression System

机译：异源毒素剪裁和转运蛋白在异源氰基毒素表达系统中的诱变

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The microcystins are a large group of cyclic peptide hepatotoxins produced by several genera of freshwater cyanobacteria. The genes responsible for microcystin biosynthesis are encoded within a large (similar to 55 kbp) gene cluster, mcyA-J. The recent establishment of a cyanotoxin heterologous expression system in Escherichia coli has provided the means to study microcystin biosynthesis in a genetically tractable, rapidly growing host. Using this system, we demonstrate that deletion of the ABC-transporter, mcyH, and dehydrogenase, mcyI, abolishes microcystin production, while deletion of the O-methyl-transferase, mcyJ, results in the production of the demethylated (DM) toxin [D-Asp(3), DMAdda(5)]microcystin-LR Both methylated and DM toxin variants were heterologously produced at high titers and efficiently exported into the extracellular medium, enabling easy purification. The results show that the mcy gene cluster can be engineered in E. coli to study the function of its individual components and direct the synthesis of particular microcystin variants. This technology could potentially be applied to other natural products of ecological and biomedical significance.
机译：微囊辛是由几个淡水蓝藻产生的一大群循环肽肝毒素。负责微囊藻生物合成的基因在大（类似于55kbp）基因簇中，mcya-j进行编码。最近在大肠杆菌中建立青紫酸毒素异源表达系统，提供了在遗传般的快速生长宿主中研究微囊藻生物合成的方法。使用该系统，我们证明了ABC-Transporter，Mcyh和脱氢酶，Mcyi，消除了微囊藻酰胺的产生，同时缺失O-甲基转移酶McyJ，导致脱甲基化（DM）毒素的产生[D. -asp（3），DMADDA（5）]甲基化和DM毒素变体的微囊糖素-LR在高滴度下异源产生，有效地出口到细胞外介质中，使易于纯化。结果表明，MCY基因簇可以在大肠杆菌中设计，以研究其各个组分的功能并引导特定的微囊藻变体的合成。这项技术可能适用于生态和生物医学意义的其他天然产物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号