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首页> 外文期刊>Crystallography reports >Crystallization and X-Ray Diffraction Studies of Salmonella Invasion Protein D (SipD), Insight Tip Component of Salmonella typhi Type III Secretion System
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Crystallization and X-Ray Diffraction Studies of Salmonella Invasion Protein D (SipD), Insight Tip Component of Salmonella typhi Type III Secretion System

机译:沙门氏菌侵袭蛋白D(SIPD)的结晶和X射线衍射研究,沙门氏菌Typhi III型分泌系统的见解尖端组分

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摘要

Many pathogenic gram-negative bacteria including Salmonella typhi utilize type III secretion systems (T3SSs) to inject bacterial proteins into host cells and to initiate infections. One of the essential components of the T3SS is the tip protein known as SipD, translocon component via the type III secretion system SPI-1. Here, recombinant SipD is produced and crystallized; the details of cloning, expression, and crystallization are reported. Crystals of SipD are obtained by sitting-drop vapour diffusion method. X-ray diffraction data are collected up to 3.8 angstrom resolution. Single crystals belong to the monoclinic sp. gr. P2; unit-cell parameters: a = 70.86, b = 103.70, c = 89.28 angstrom, beta = 107.63 degrees.
机译:许多致病革兰氏阴性细菌,包括沙门氏菌Typhi使用III型分泌系统(T3SS)将细菌蛋白注射到宿主细胞中并引发感染。 T3SS的一个基本组分是称为SIPD的尖端蛋白,通过III型分泌系统SPI-1称为SIPD。 在此,产生重组SIPD并结晶; 报道了克隆,表达和结晶的细节。 SIPD的晶体通过坐下的蒸汽扩散方法获得。 收集X射线衍射数据高达3.8埃分辨率。 单晶属于单斜晶状物。 GR。 P2; 单位单元参数:a = 70.86,b = 103.70,c = 89.28埃,β= 107.63度。

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