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On the Mechanism of Membrane Protein Crystallization in Lipidic Mesophases

机译：脂质中间相中膜蛋白结晶的机理

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The in meso method for crystallizing membrane proteins has been shown to work with a range of different protein types. The method involves reconstituting the target protein into the bilayer of a bicontinuous lipid mesophase followed by an induced phase separation brought on by the addition of a precipitant. A mechanism has been proposed for how in meso crystallogenesis comes about at a molecular level. However, aspects of the hypothesis are not fully developed. These are examined and expanded on here by considering the behavior of related but simpler systems and processes where the mode of action is understood and founded in experiment. By building up from this series of model, well behaved systems a more complete view of what takes place during the in meso crystallogenesis of membrane proteins is obtained. In addition to the underlying theory, some of the more practical aspects of the method are explored and their basis in the hypothesis is established. This treatment suggests a host of experiments to further test and develop the hypothesis. By deciphering the underlying molecular mechanism, clearer insights emerge for practicing a rational approach to membrane protein crystallogenesis.
机译：已经证明结晶膜蛋白的内观方法可用于多种不同的蛋白类型。该方法包括将靶蛋白重构到双连续脂质中间相的双层中，然后通过添加沉淀剂进行诱导的相分离。对于介观结晶如何在分子水平上发生的机制已经提出。但是，该假设的各个方面尚未得到充分发展。通过考虑相关但更简单的系统和过程的行为来检查和扩展这些行为，在实验中理解并建立了作用方式。通过建立该系列模型，行为良好的系统，可以更全面地了解膜蛋白的介观结晶过程。除了基本理论之外，还探讨了该方法的一些更实际的方面，并在假设中建立了它们的基础。这种处理方法提出了许多实验，以进一步检验和发展这一假设。通过解释潜在的分子机制，对于实践合理的膜蛋白结晶发生方法，人们有了更清晰的认识。

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