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A meta-analysis of the expression of HaCRE1, a young Copia LTR-retrotransposon of sunflower (Helianthus annuus L.)

机译:对向日葵(Helianthus Annuus L.)的年轻Copia Ltr-Retrotransposon的HACRE1表达的荟萃分析(Helianthus Annuus L.)

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摘要

The expression level of HaCREl, a sunflower long-terminal-repeats retrotransposon which was shown to be inserted into the genome in relatively recent times was studied in detail in order to evaluate its mutagenic potential. A meta-analysis was performed, using cDNA libraries available from public repositories including cDNA sequences isolated from roots of sunflower plants treated with different plant hormones, NaCl and polyethylene glycol. HaCREl was expressed at low level under all treatments and even in the control roots, compared to sunflower gene sequences. Only abscisic acid treatment induced over-expression of the retrotransposon. The putative cis-regulatory sequences responsible for HaCREl transcription were identified in its 5'-long-terminal repeat. It was concluded that, even at low level, this still "young" retrotransposon maintains its capacity to transpose and hence its mutagenic potential.
机译:详细研究了在相对近期插入到基因组中的向日葵长末端重复转回转突骨的褐发制的表达水平,以评估其致突变性的潜力。 使用公共储存库中提供的cDNA文库进行META分析,包括用不同植物激素,NaCl和聚乙二醇处理的向日葵植物的根源分离的cDNA序列。 与向日葵基因序列相比,在所有治疗中,均匀在较低水平下表达藻类,甚至在控制根部。 只有脱落酸治疗诱导转回横向的过度表达。 在其5'-长末端重复中鉴定了负责黑粉族转录的推定的顺式调节序列。 结论是,即使在低水平下,这仍然是“年轻”Retrotransposon保持其传输能力,从而保持其致突变性的潜力。

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