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首页> 外文期刊>Alcohol >Voluntary elevated ethanol consumption in adolescent Sprague-Dawley rats: Procedural contributors and age-specificity
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Voluntary elevated ethanol consumption in adolescent Sprague-Dawley rats: Procedural contributors and age-specificity

机译：青少年Sprague-Dawley大鼠自愿升高的乙醇消费：程序贡献者和年龄特异性

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Alcohol consumption is typically initiated during adolescence, with the incidence of binge drinking (production of blood ethanol concentrations [BECs] > 80 mg/dL) peaking during this stage of development. Studies in outbred rats investigating the consequences of adolescent ethanol exposure have typically employed intragastric, vapor, or intraperitoneal administration to attain BECs in this range. While these procedures have yielded valuable data regarding the consequences of adolescent exposure, they are varyingly stressful, administer the full dose at once, and/or bypass digestion. Consequently, we have worked to develop a model of voluntary elevated ethanol consumption in outbred adolescent Sprague-Dawley males and females, building on our previous work (see Hosova & Spear, 2017). This model utilizes daily 30-min access to 10% ethanol (v/v) in chocolate Boost from postnatal day (P)28-41. Experiment 1 compared intake levels between (1a) animals given either ball-bearing or open-ended sipper tube tips for solution access, (1b) animals separated from their cage mate by wire mesh or isolated to a separate cage during solution access, (1c) animals given solution access with or without simultaneous access to banana-flavored sugar pellets, and (1d) animals that were either moderately food-restricted or fed ad libitum. Experiment 2 compared intake levels between animals given daily solution access and animals given access only on a "Monday-Wednesday-Friday" intermittent schedule. Experiment 3 compared adolescent and adult (P70-83) consumption using the finalized procedure as based on the results of Experiments 1 and 2. As in our previous work, consumptions well within the binge range were produced on some days, with high-consumption days typically followed by several days of lower consumption before increasing again. Sipper tube type (la) and simultaneous pellet access (1c) did not affect consumption, while intake was significantly higher in non-isolated (1b), food restricted (id), daily-access (2), and adolescent (3) animals. However, although ethanol intake was higher in food-restricted animals, the resulting BECs were equivalent or higher in non-restricted animals, likely due to a hepatoprotective effect of moderate food restriction. Post-consumption intoxication ratings correlated with BECs and were notably higher in adults than adolescents, despite the lower voluntary consumption levels of adults, confirming prior reports of the attenuated sensitivity of adolescents to ethanol intoxication relative to adults. The final model utilized ball-bearing sipper tube tips to provide daily access to 10% ethanol in chocolate Boost to free-feeding adolescent animals separated from their cage mate by wire mesh, with no food provided during solution access. This easy-to implement model is effective in producing elevated voluntary ethanol consumption in adolescent, but not adult, Sprague-Dawley rats. (C) 2019 Elsevier Inc. All rights reserved.
机译：醇消耗通常在青春期期间发起，随着在这种发育阶段的峰值期间，狂暴饮用的发病率（血液乙醇浓度[BECS]> 80mg / dL）。研究青少年乙醇暴露的后果的异常大鼠的研究通常使用胃内，蒸气或腹膜内给药来获得该范围内的BEC。虽然这些程序已经产生了有关青少年暴露后果的有价值的数据，但它们是变化的压力，一次性给药，和/或旁路消化。因此，我们曾致力于在我们以前的工作中建立繁殖青少年Sprague-Dawley男性和女性的乙醇消费模型（参见Hosova＆Spear，2017）。该模型在后期（P）28-41中，每天30分钟获得巧克力升压的10％乙醇（v / v）。实验1在给定滚珠或开放式的Sipper管尖端的（1A）动物之间的摄入水平用于溶液进入，（1B）通过丝网与笼式伴侣分离的动物或在溶液进入期间分离为单独的笼，（1C ）通过同时使用或不同时进入香蕉风味的糖颗粒的溶液进入，以及（1D）动物，这些动物受到适度的食物限制或喂养自由的动物。实验2在每日解决方案访问和动物的动物之间比较仅在“周一至周三 - 星期五”间歇计划中获得的动物。实验3比较青少年和成人（P70-83）使用最终过程的消耗量为基于实验1和2.如我们以前的工作，在某些日子里产生偏风范围内的消费良好，具有高消耗天通常在再次增加之前之后的几天较低的消耗。 Sipper Tube型（La）和同时颗粒接入（1c）没有影响消耗，而非隔离（1b），食物限制（ID），每日接入（2）和青少年（3）动物的摄入量显着高。 。然而，虽然食物限制动物的乙醇摄入量较高，但是由于中度食物限制的肝脏保护作用，所得BEC在非受限制的动物中是等同的或更高的。消耗后的中毒评级与BEC相关，并且成年人的成年人具有显着高，尽管成年人的自愿消费水平降低，但确认了与成年人相对于乙醇中毒的减毒敏感性的报道。最终模型利用了滚珠轴承管提示，以便每天获得10％乙醇的巧克力升压，以通过丝网与笼式伴侣分离的自由喂食青少年动物，在溶液进入期间没有提供食物。这种易于实现的模型在产生青少年的升高的自愿乙醇消费方面是有效的，但不是成年人的Sprague-Dawley大鼠。 （c）2019 Elsevier Inc.保留所有权利。

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