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首页> 外文期刊>Crystal growth & design >Precipitant-Controlled Growth of Lysozyme Crystals in Sodium Thiocyanate
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Precipitant-Controlled Growth of Lysozyme Crystals in Sodium Thiocyanate

机译：硫氰酸钠中溶菌酶晶体的沉淀控制生长

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We have shown previously that large high-quality single protein crystals can be grown by varying temperature in a predetermined fashion such that the solution supersaturation and the rate of crystal growth are maintained at a constant value in batch crystallization systems.Here we have adapted the constant supersaturation control(CSC)methodology to isothermal crystal growth.In this version of the CSC protocol,dynamic control is provided by changing precipitant concentration through a dialysis membrane.We have developed a system of multiple growth cells in series that allows a variety of precipitant concentration trajectories to be sampled simultaneously during a single CSC experiment.Several different concentration profiles were used to grow lysozyme crystals in NaSCN salt.Increasing the precipitant concentration using a slow linear profile results in the highest quality crystals while requiring the least amount of characterization work.Coupling this type of profile with multiple growth cells in series appears to be a promising method for dynamically controlling protein crystal growth isothermally.
机译：先前我们已经表明，可以通过以预定方式改变温度来生长大型高质量的单蛋白晶体，从而在分批结晶系统中将溶液过饱和度和晶体生长速率保持在恒定值。等温晶体生长的超饱和控制（CSC）方法。在此版本的CSC协议中，通过通过透析膜改变沉淀剂浓度来提供动态控制。我们开发了一系列串联的多个生长池的系统，该系统允许各种沉淀剂浓度在单个CSC实验中要同时采样轨迹，使用几种不同的浓度曲线在NaSCN盐中生长溶菌酶晶体，使用缓慢的线性曲线增加沉淀剂的浓度可以得到最高质量的晶体，而所需的表征工作最少。这种类型的配置文件有多个增长h细胞串联似乎是一种有前途的方法来等温地动态控制蛋白质晶体的生长。

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