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Dynamic screening experiments to maximize hits for crystallization

机译：动态筛选实验可最大化命中结晶

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摘要

In the first step of crystallization screening, the protein is exposed. to a wide variety of reagents at different concentrations. Once a "hit" deemed to be conducive to crystallization is identified, parameters such as precipitant concentration, pH, and temperature are used to produce crystals suitable for analysis by X-ray diffraction. Crystals, crystalline precipitate, and phase separation are usually considered leads that are worth pursuing. Clear drops are mostly disregarded. This paper presents a screening technique that makes use of clear drops. Clear drops are subjected to evaporation with the aim of driving them to supersaturation. The findings reported bring a new dimension to screening and open up the scope for utilizing a potential wealth of crystallization conditions that are currently being ignored. Furthermore, this technique enables the utilization of far less protein sample and allows us to obtain the hits in shorter times.
机译：在结晶筛选的第一步中，将蛋白质暴露出来。各种浓度不同的试剂。一旦确定了认为有助于结晶的“击中”，就使用诸如沉淀剂浓度，pH和温度之类的参数来生产适用于X射线衍射分析的晶体。晶体，晶体沉淀物和相分离通常被认为是值得追求的铅。晴滴大多被忽略。本文提出了一种利用透明液滴的筛选技术。透明的液滴会蒸发，以使其达到过饱和状态。报告的发现为筛选和开拓利用潜在的大量结晶条件的范围开辟了新的领域，而这些结晶条件目前被忽略。此外，该技术可以利用更少的蛋白质样品，并允许我们在更短的时间内获得匹配。

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