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首页> 外文期刊>Acta neurobiologiae experimentalis >Effect of stress on the expression of GnRH and GnRH receptor (GnRH-R) genes in the preoptic area-hypothalamus and GnRH-R gene in the stalk/median eminence and anterior pituitary gland in ewes during follicular phase of the estrous cycle.
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Effect of stress on the expression of GnRH and GnRH receptor (GnRH-R) genes in the preoptic area-hypothalamus and GnRH-R gene in the stalk/median eminence and anterior pituitary gland in ewes during follicular phase of the estrous cycle.

机译：应激对发情周期卵泡期卵前/下丘脑中GnRH和GnRH受体（GnRH-R）基因表达的影响，茎/中位隆突和垂体前腺的GnRH-R基因表达。

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The RT-PCR (reverse transcription polymerase chain reaction) technique was used to analyze GnRH mRNA and GnRH-R mRNA in the preoptic area, anterior and ventromedial hypothalamus, and GnRH-R mRNA in the stalk/median eminence and anterior pituitary gland of follicular ewes subjected to short (3 h during one day) or prolonged (5 h daily during four consecutive days) footshock stimulation. To analyze relationship between expression of GnRH and GnRH-R genes with LH secretion the blood samples were collected at 10 min intervals to determine LH levels in control and stressed animals. The concentration of GnRH mRNA increased significantly in the preoptic area, anterior and ventromedial hypothalamus of ewes subjected to short stress. The prolonged stressful stimuli significantly decreased GnRH mRNA levels in all analyzed structures. In short stressed ewes the significant augmentation of mRNA encoding GnRH-R was detected in the preoptic area, entire hypothalamus, stalk/median eminence and anterior pituitary gland. The GnRH-R mRNA was significantly reduced in all tested structures of animals subjected to prolonged footshocking except for the preoptic area, where GnRH-R mRNA did not differ from control values. The changes in GnRH mRNA and GnRH-R mRNA levels under short or prolonged stress were associated with an increase or decrease of LH concentration in blood plasma, suggesting the existence of a direct relationship between GnRH mRNA and GnRH-R mRNA expression with LH secretion. The results indicate that the expression of both GnRH gene and GnRH-R gene, as well as LH secretion in ewes during the follicular phase of the estrous cycle, are dependent upon the kind of stress.
机译：RT-PCR（逆转录聚合酶链反应）技术用于分析卵泡前视区，前下丘脑和腹膜下丘脑的GnRH mRNA和GnRH-R mRNA以及茎/中部隆起和垂体前叶腺的GnRH-R mRNA短时间（一天3小时）或长时间（连续四天每天5小时）刺激的母羊。为了分析GnRH和GnRH-R基因表达与LH分泌之间的关系，每隔10分钟收集一次血样，以确定对照组和应激动物的LH水平。 GnRH mRNA的浓度在受到短压力的母羊的视前区，前丘脑和下丘脑下丘脑中显着增加。长时间的压力刺激显着降低了所有分析结构中的GnRH mRNA水平。简而言之，在视前区，整个下丘脑，茎/中部隆起和垂体前叶腺体中检测到编码GnRH-R的mRNA的显着增强。在所有接受长时间持续电击的动物测试结构中，GnRH-R mRNA均显着降低，除了视前区（GnRH-R mRNA与对照值没有差异）外。短期或长期应激下GnRH mRNA和GnRH-R mRNA水平的变化与血浆中LH浓度的升高或降低有关，表明GnRH mRNA和GnRH-R mRNA表达与LH分泌之间存在直接关系。结果表明，在发情周期的卵泡期，GnRH基因和GnRH-R基因的表达以及母羊的LH分泌均取决于应激的种类。

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