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首页> 外文期刊>American journal of dentistry >Effect of biofilm exposure on marginal integrity of composite restorations
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Effect of biofilm exposure on marginal integrity of composite restorations

机译：生物膜暴露对复合修复的边缘完整性的影响

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Purpose: To evaluate the effect of bacterial exposure on the marginal integrity of dentin-resin interfaces for composites with and without bioactive glass (BAG). Methods: Cavity preparations of 5 mm width and 1.5 mm depth were machined into dentin disks by means of a computer controlled milling system. After applying the bonding agent, cavity preparations (n=3-5) were restored by incremental technique with experimental resin composites (50:50 BisGMA/TEGDMA: 72wt% filler) with different filler compositions: control - 67 wt% silanated strontium glass and 5wt% aerosol-silica filler and BAG - 57 wt% silanated strontium glass and 15 wt% BAG-65 wt% silica. Samples were then stored in sterile Todd-Hewitt media or co-incubated with Streptococcus mutans (UA 159), at 37 degrees C, 5% CO2 for 1-2 weeks. For samples co-incubated with a living biofilm, a luciferase assay was performed in order to assess its viability. Surfaces were impressed before and after each storage condition and replicas examined in a scanning electron microscope. Using image analysis software (Image J), the discontinuous margins percentage (%DM) was quantitatively assessed. Data were analyzed using two-way ANOVA followed by Tukey's test (alpha= 0.05). Results: Gap size ranged between 7-23 mu m. The bacterial exposure significantly increased the %DM in both groups predominantly due to the formation of new gap regions. There was no difference between control and BAG composites regarding %DM and the biofilm viability. Bacterial exposure promoted degradation of composite restoration marginal integrity, with no difference between composites with and without BAG.
机译：目的：评价细菌暴露对具有和不具有生物活性玻璃（袋）复合材料的牙本质树脂界面的边际完整性的影响。方法：通过计算机控制铣削系统加工5mm宽度和1.5mm深度的腔制剂。施用粘合剂后，通过具有不同填料组合物的实验树脂复合材料（50:50 bisgma / Tegdma：72wt％填料）的增量技术来恢复腔制剂（n = 3-5）：对照 - 67wt％硅烷锶玻璃和5wt％气溶胶 - 二氧化硅填料和袋 - 57wt％硅烷锶玻璃和15wt％袋-65wt％二氧化硅。然后将样品储存在无菌ToDD-Hewitt介质中，或与链球菌（UA 159）共孵育，在37℃，5％CO 2中孵育1-2周。对于与活生物膜共孵育的样品，进行荧光素酶测定以评估其活力。在扫描电子显微镜中检查的每个储存条件和复制品之前和之后，留下表面深刻。使用图像分析软件（图像j），定量评估不连续的边距百分比（％dm）。使用双向ANOVA进行分析数据，然后进行Tukey的测试（alpha = 0.05）。结果：间隙尺寸范围为7-23亩。由于新的间隙区域的形成，细菌暴露在两组中的两组中的％DM显着增加。对价值％DM和生物膜活力之间的控制和袋复合材料之间没有差异。细菌曝光促进复合恢复边缘完整性的降解，复合材料无差异，没有袋子。

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