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首页> 外文期刊>Crystal growth & design >Tooth enamel proteins enamelin and amelogenin cooperate to regulate the growth morphology of octacalcium phosphate crystals
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Tooth enamel proteins enamelin and amelogenin cooperate to regulate the growth morphology of octacalcium phosphate crystals

机译：牙釉质蛋白enamelin和amelogenin共同调节磷酸八钙晶体的生长形态

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摘要

To examine the hypothetical cooperative role of enamelin and amelogenin in controlling the growth morphology of enamel crystals in the postsecretory stage, we applied a cation selective membrane system for the growth of octacalcium phosphate (OCP) in the truncated recombinant porcine amelogenin (rP148) with and without the 32 kDa enamelin fragment. Enamelin alone inhibited the growth in the c-axis direction more than rP148, yielding OCP crystals with the smallest aspect ratio of all conditions tested. When enamelin was added to the amelogenin "gel-like matrix", the inhibitory action of the protein mixture on the growth of OCP in the c-axis direction was diminished, while that in the b-axis direction was increased. As a result, the length to width ratio (aspect ratio) of OCP crystal was markedly increased. Addition of enamelin to amelogenin enhanced the potential of amelogenin to stabilize the amorphous calcium phosphate (ACP) transient phase. The ratio of enamelin and amelogenin was crucial for stabilization of ACP and the growth of OCP crystals with larger aspect ratio. The cooperative regulatory action of enamelin and amelogenin was attained, presumably, through coassembling of enamelin and amelogenin. These results have important implications in understanding the growth mechanism of enamel crystals with large aspect ratio.
机译：为了检验假想蛋白和牙釉蛋白在分泌后阶段控制牙釉质晶体生长形态的假设协同作用，我们应用阳离子选择性膜系统在截短的重组猪牙釉蛋白（rP148）中磷酸八钙（OCP）的生长，并与没有32 kDa的enamelin片段。单独的Enamelin抑制c轴方向的生长的作用大于rP148，从而产生了在所有测试条件下纵横比最小的OCP晶体。当向釉原蛋白“凝胶样基质”中加入釉蛋白时，蛋白质混合物对OCP在c轴方向上的生长的抑制作用减弱，而在b轴方向上的抑制作用增加。结果，OCP晶体的长宽比（纵横比）显着增加。在釉原蛋白中加入脑釉蛋白可增强釉原蛋白稳定无定形磷酸钙（ACP）瞬态相的潜力。 Enamelin和amelogenin的比例对于稳定ACP和长宽比较大的OCP晶体的生长至关重要。据推测，通过将Enamelin和Amelogenin共同组装，可以实现Enamelin和Amelogenin的协同调节作用。这些结果对理解大纵横比的搪瓷晶体的生长机理具有重要意义。

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