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首页> 外文期刊>Acta Neurochirurgica >Effect of erythrocytes on brain water content and haem oxygenase-1 expression in rats with traumatic intracerebral haemorrhage
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Effect of erythrocytes on brain water content and haem oxygenase-1 expression in rats with traumatic intracerebral haemorrhage

机译：红细胞对创伤性脑出血大鼠脑含水量和血红素氧化酶-1表达的影响

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Background: Studies have demonstrated that brain oedema formation following spontaneous intracerebral haemorrhage is associated with substances derived from blood clots or blood components. However, these studies did not completely reveal the role of blood components in brain oedema formation following traumatic intracerebral haemorrhage (TICH). Here, we explore the role of erythrocytes in brain oedema development by studying the effect of erythrocytes on brain water content (BWC) and expression of haem oxygenase-1 (HO-1) in rats with TICH. Methods: A total of 120 Sprague-Dawley rats were randomly divided into four experimental treatment groups: traumatic brain injury (TBI), TBI plus whole blood (WB), TBI plus lysed red blood cells (RBCs; LRBC) and TBI plus packed RBCs (PRBC). Following TBI, which was established by applying a free-falling device, WB, LRBC or PRBC were infused with stereotactic guidance into the injured cortex to produce a model of TICH. All rats were killed at 1, 3 or 5 days after TBI or TICH. BWC was measured, and immunohistochemistry for HO-1 was performed. Results: In the WB, PRBC and TBI groups, BWC at 3 days post-TBI or post-TICH was the greatest. However, BWC in the LRBC group at 1 day was markedly higher than that at 3 and 5 days. Comparisons among the four groups showed that BWC in the LRBC group was the highest at 1 day, and the highest at 3 days in the WB and PRBC groups; there was no significant difference at 5 days. Positive expression of HO-1 in the WB, PRBC and LRBC groups coincided with changes in BWC. Conclusions: Our results indicate that erythrocytes play an important role in delayed brain oedema formation (3 days post-injury) following TICH, but have no significant influence on brain oedema at early stages (1 day post-injury), and that the mechanisms of delayed brain oedema involve RBC breakdown products.
机译：背景：研究表明，自发性脑出血后脑水肿的形成与血栓或血液成分有关。但是，这些研究并未完全揭示血液成分在颅内出血（TICH）后脑水肿形成中的作用。在这里，我们通过研究红细胞对TICH大鼠脑水含量（BWC）和血红素加氧酶1（HO-1）表达的影响，探讨了红细胞在脑水肿发展中的作用。方法：将120只Sprague-Dawley大鼠随机分为四个实验治疗组：脑外伤（TBI），TBI加全血（WB），TBI加裂解性红细胞（RBC; LRBC）和TBI加包装红细胞（PRBC）。在通过使用自由落体装置建立的TBI之后，将WB，LRBC或PRBC与立体定向引导一起注入受伤的皮层中，以生成TICH模型。在TBI或TICH后1、3或5天处死所有大鼠。测量BWC，并进行HO-1的免疫组织化学。结果：在WB，PRBC和TBI组中，TBI后或TICH后3天的BWC最大。但是，LRBC组在1天时的BWC明显高于3天和5天时的BWC。四组之间的比较表明，LRBC组的BWC在1天时最高，而WB和PRBC组在3天时最高。 5天无明显差异。 WB，PRBC和LRBC组中HO-1的阳性表达与BWC的变化相吻合。结论：我们的结果表明，红细胞在TICH后延迟脑水肿形成（损伤后3天）中起重要作用，但对早期（损伤后1天）脑水肿没有显着影响，并且其机制延迟性脑水肿涉及RBC分解产物。

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