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首页> 外文期刊>Allergy >Features of mesenchymal transition in the airway epithelium from chronic rhinosinusitis
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Features of mesenchymal transition in the airway epithelium from chronic rhinosinusitis

机译：慢性鼻窦炎气道上皮中间充质转换的特征

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摘要

Conclusion Features of epithelial dedifferentiation towards a mesenchymal phenotype are observed in CRSwNP and CRSsNP and correlate with airway fibrosis and inflammation.Background Chronic rhinosinusitis (CRS) defines a group of disorders characterized by persistent inflammation of the sinonasal tract. Epithelial changes and structural remodelling are present, but whether epithelial differentiation is altered remains uncertain.Methods To evaluate the differentiation state of the sinonasal epithelium in CRS, sinonasal biopsies from patients with CRS with nasal polyps (CRSwNP) or CRS without nasal polyps (CRSsNP), or with allergic rhinitis (AR), as compared to controls, were processed by immunohistochemistry and RT-qPCR for terminal differentiation (E-cadherin, high molecular weight cytokeratins (Hmw CK) and CK5, vimentin) and lineage differentiation (?-tubulin IV+ ciliated cells, MUC5AC+ goblet cells, p63 + basal cells). Findings were correlated with subepithelial fibrosis and clinical CT score.Results Expression of E-cadherin was decreased at protein and mRNA levels in CRSwNP and CRSsNP, as compared to controls. Staining for Hmw CKs was also reduced in CRSwNP and CRSsNP, and CK5 mRNA was decreased in CRSwNP. These features were not due to changes in lineage specification, but associated with increases in vimentin-expressing epithelial cells. In addition, vimentin expression correlated with the basement membrane thickening and with CT score, as well as with tissue eosinophils.
机译：结论在CRSPNP和CRSSNP中观察到间充质表型上皮消除功能的特征，并与气道纤维化和炎症相关.Backgroung慢性鼻窦炎（CRS）定义了一组疾病，其特征在于Sinonasal道的持续炎症。存在上皮变化和结构重塑，但是上皮分化是否改变仍然不确定。评估CRS中Sinonasal上皮的分化状态，从鼻息肉（CRSWNP）或CRS的CRS患者中的Sinonasal活检，Sinonasal活组织检查，没有鼻息肉（CRSSNP）与对照相比，通过免疫组织化学和RT-QPCR来加工末端分化（E-Cadherin，高分子量细胞疫（HMW CK）和CK5，Vimentin）和谱系分化（α-丁蛋白）（β-丁蛋白IV +纤毛细胞，MUC5AC +脚卷细胞，P63 +基础细胞）。结果与耻骨纤维化和临床CT得分相关。与对照相比，CRSMNP和CRSSNP中的蛋白质和mRNA水平降低了E-Cadherin的表达。在CRSWNP和CRSSNP中也降低了HMW CKS的染色，CK5 mRNA在CRSPNP中降低。这些特征不是由于谱系统规范的变化，但随着试剂表达的上皮细胞的增加相关。此外，Vimentin表达与基底膜增稠和CT分数以及组织嗜酸性粒细胞相关。

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  • 来源
    《Allergy》 |2014年第11期|共10页
  • 作者

    HupinC.; GohyS.; BouzinC.; LecocqM.; PoletteM.; PiletteC.;

  • 作者单位

    Pole de Pneumologie ORL and Dermatologie Institut de Recherche Expérimentale et Clinique (IREC);

    Pole de Pneumologie ORL and Dermatologie Institut de Recherche Expérimentale et Clinique (IREC);

    Imaging Platform Institut de Recherche Expérimentale et Clinique (IREC) Université Catholique de;

    Pole de Pneumologie ORL and Dermatologie Institut de Recherche Expérimentale et Clinique (IREC);

    SFR CAP-Santé INSERM UMR-S 903 University of Reims-Champagne-ArdenneReims France;

    Pole de Pneumologie ORL and Dermatologie Institut de Recherche Expérimentale et Clinique (IREC);

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医学免疫学;
  • 关键词

    airway fibrosis; epithelial-to-mesenchymal transition; rhinosinusitis; vimentin;

    机译：气道纤维化;上皮 - 间充质转换;鼻窦炎;波形蛋白;

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