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首页> 外文期刊>Allergy >Identification of a polygalacturonase (Cup s 2) as the major CCD-bearing allergen in Cupressus sempervirens pollen
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Identification of a polygalacturonase (Cup s 2) as the major CCD-bearing allergen in Cupressus sempervirens pollen

机译：鉴定聚氨酯酶（CUP S 2）作为Cupressus sempervirens花粉中的主要CCD轴承过敏原

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As IgE glyco-epitopes, also referred to as cross-reactive carbohydrate determinants (CCDs), can share significant structural homologies between different plants, they are prone to extensive cross-reactivity among allergen pollen extracts. Here, cypress pollen allergens, especially a polygalacturonase (PG), were further characterized using double one-dimensional electrophoresis (D1-DE). The presence of specific IgE directed against CCDs was investigated by bromelain IgE inhibition and concanavalin A binding assays using sera of cypress pollen-sensitized patients. Our results showed that IgE reactivity to CCDs in Cupressus sempervirens pollen extracts is mainly related to bromelain-type epitopes of a newly identified cypress PG. This glycoprotein has been further characterized through an immunoproteomic approach and officially indexed as Cup s 2 by the WHO/IUIS allergen nomenclature. Cup s 2 could thus be associated with the increased prevalence of IgE reactivity to cypress pollen extracts because of CCD interference.
机译：作为IgE甘油表位，也称为交叉反应性碳水化合物测定法（CCD），可以在不同植物之间共享显着的结构同源物，它们在过敏原花粉提取物之间容易出现广泛的交叉反应性。这里，使用双一维电泳（D1-de）进一步表征柏树花粉过敏原，尤其是聚乳糖酰酶（PG）。通过溴elige抑制和康乃伊抑制患者的血清血清血清抑制和康丹林，研究了针对CCD的特异性IgE的存在。我们的研究结果表明，Cupressus Sempervirens花粉提取物对CCD的IgE反应性主要与新鉴定的柏树PG的菠萝蛋白型表位有关。该糖蛋白通过免疫蛋白质方法进一步表征，并通过WHO / IUIS过敏原命名法正式编制为杯S 2。因此，由于CCD干扰，杯S 2可以与IgE反应性与柏树花粉提取物的流行增加相关。

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