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首页> 外文期刊>ACS Macro Letters >Digital Maskless Photolithographic Patterning of DNA-Functionalized Poly(ethylene glycol) Diacrylate Hydrogels with Visible Light Enabling Photodirected Release of Oligonucleotides
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Digital Maskless Photolithographic Patterning of DNA-Functionalized Poly(ethylene glycol) Diacrylate Hydrogels with Visible Light Enabling Photodirected Release of Oligonucleotides

机译：DNA官能化聚（乙二醇）二丙烯酸酯水凝胶的数字掩模光刻图案化，具有可见光，使寡核苷酸的光电二核苷酸释放

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摘要

Soft biomaterials possessing structural hierarchy have growing applications in lab-on-chip devices, artificial tissues, and micromechanical and chemomechanical systems. The ability to integrate sets of biomolecules, specifically DNA, within hydrogel substrates at precise locations could offer the potential to form and modulate complex biochemical processes with DNA-based molecular switches in such materials and provide a means of creating dynamic spatial patterns, thus enabling spatiotemporal control of a wide array of reaction-diffusion phenomena prevalent in biological systems. Here we develop a means of photopatterning two-dimensional DNA-functionalized poly(ethylene glycol) diacrylate (PEGDA) hydrogel architectures with an aim toward these applications. While PEGDA photopatterning methods are well-established for the fabrication of hydrogels, including those containing oligonucleotides, the photoinitiators typically used have significant crosstalk with many UV-photoswitchable chemistries including nitrobenzyl derivatives. We demonstrate the digital photopatterning of PEGDA-co-DNA hydrogels using a blue light-absorbing (470 nm peak) photoinitiator system and macromer comprised of camphorquinone, triethanolamine, and poly(ethylene glycol) diacrylate (M-n = 575) that minimizes absorption in the UV-A wavelength range commonly used to trigger photoswitchable chemistries. We demonstrate this method using digital maskless photolithography within microfluidic devices that allows for the reliable construction of multidomain structures. The method achieves feature resolutions as small as 25 mu m, and the resulting materials allow for lateral isotropic bulk diffusion of short single-stranded (ss) DNA oligonucleotides. Finally, we show how the use of these photoinitiators allows for orthogonal control of photopolymerization and UV-photoscission of acrylate-modified DNA containing a 1-(2-nitrophenyl) ethyl spacer to selectively cleave DNA from regions of a PEGDA substrate.
机译：具有结构层次结构的软生物材料在芯片片装置，人工组织和微机械和化学机械系统中具有越来越多的应用。在精确位置的水凝胶基材中整合一组生物分子（特别是DNA）的能力可以在这种材料中具有与基于DNA的分子开关形成和调节复杂的生物化学过程，并提供产生动态空间模式的手段，从而实现时尚的手段控制生物系统中普遍的各种反应扩散现象。在这里，我们开发了Pherpatterning的二维DNA官能化聚（乙二醇）二丙烯酸酯（PEGDA）水凝胶架构的手段，目的是朝向这些应用。虽然PEGDA Phertopatterning方法是用于制备水凝胶的良好的，但包括含寡核苷酸的那些，通常使用的光引发剂具有显着的串扰，其具有许多UV-光学性化学物质，包括硝基苄基衍生物。我们展示了使用由樟脑醌，三乙醇胺和聚（乙二醇）二丙烯酸酯（Mn = 575）组成的蓝色光吸收（470nm峰）光引发剂体系和大分子蛋白的粘合剂 - 共DNA水凝胶的数字照射。最小化吸收紫外线 - 一个常用的波长范围，用于触发光学性化学。我们在微流体装置内使用数字掩模光刻来证明该方法，其允许多畴结构的可靠构造。该方法实现了小于25μm的特征分辨率，并且所得材料允许短单链（SS）DNA寡核苷酸的横向各向同性散装扩散。最后，我们展示了这些光引发剂的使用方式允许含有1-（2-硝基苯基）乙基间隔物的丙烯酸酯改性DNA的光聚合和UV-PhotoScionse的正交控制，以从PEGDA底物的区域中选择性地切割DNA。

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  • 来源
    《ACS Macro Letters》 |2019年第9期|共8页
  • 作者

    Dorsey Phillip J.; Rubanov Moshe; Wang Wenlu; Schulman Rebecca;

  • 作者单位

    Johns Hopkins Univ Dept Chem &

    Biomol Engn 3400 North Charles St Baltimore MD 21218 USA;

    Johns Hopkins Univ Dept Chem &

    Biomol Engn 3400 North Charles St Baltimore MD 21218 USA;

    Johns Hopkins Univ Dept Chem &

    Biomol Engn 3400 North Charles St Baltimore MD 21218 USA;

    Johns Hopkins Univ Dept Chem &

    Biomol Engn 3400 North Charles St Baltimore MD 21218 USA;

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  • 正文语种 eng
  • 中图分类 有机化学;
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