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首页> 外文期刊>Animal Science Journal >Normal DNA methylation status in sperm from a somatic cell cloned bull and their fertilized embryos
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Normal DNA methylation status in sperm from a somatic cell cloned bull and their fertilized embryos

机译：来自体细胞克隆公牛的正常DNA甲基化状态和浓郁的胚胎

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摘要

Epigenetic reprogramming confers totipotency even during somatic cell nuclear transfer (SCNT), which has been used to clone various animal species. However, as even apparently healthy cloned animals sometimes have aberrant epigenetic status, the harmful effects of these defects could be passed onto their offspring. This is one of the biggest obstacles for the application of cloned animals for livestock production. Here, we investigated the DNA methylation status of four developmentally regulated genes (PEG3, XIST, OCT4, and NANOG) in sperms from a cloned and a non-cloned bull, and blastocysts obtained by invitro fertilization using those sperms and SCNT. We found no differences in the methylation status of the above genes between cloned and non-cloned bull sperms. Moreover, the methylation status was also similar in blastocysts obtained with cloned and non-cloned bull sperms. In contrast, the methylation status was compromised in the SCNT blastocysts. These results indicate that sperm from cloned bulls would be adequately reprogrammed during spermatogenesis and, thus, could be used to produce epigenetically normal embryos. This study highlights the normality of cloned bull offspring and supports the application of cloned cattle for calf production.
机译：表观遗传重新编程赋予全能性，即使在体细胞核转移（SCNT）期间也被用来克隆各种动物物种。然而，甚至显然是健康的克隆动物有时具有异常的表观遗传状态，这些缺陷的有害影响可以通过它们的后代。这是克隆动物用于牲畜生产的最大障碍之一。在这里，我们研究了来自克隆和非克隆公牛的精子的四种发育调节基因（PEG3，XIST，OCT4和NANOG）的DNA甲基化状态，以及使用这些精子和SCNT通过invitro受精获得的胚泡。我们发现克隆和非克隆牛精子之间的上述基因的甲基化状态没有差异。此外，甲基化状态在用克隆和非克隆的牛精子获得的胚泡中也相似。相反，甲基化状态在SCNT胚泡中受到损害。这些结果表明，来自克隆公牛的精子将在精子发生期间充分重新编程，因此可用于产生外观正常胚胎。本研究突出了克隆公牛后代的正常性，并支持克隆牛用于小腿生产的应用。

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