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首页> 外文期刊>Andrology >Testis-specific isoform of Na/K-ATPase (ATP1A4) regulates sperm function and fertility in dairy bulls through potential mechanisms involving reactive oxygen species, calcium and actin polymerization
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Testis-specific isoform of Na/K-ATPase (ATP1A4) regulates sperm function and fertility in dairy bulls through potential mechanisms involving reactive oxygen species, calcium and actin polymerization

机译：通过涉及反应性氧物种，钙和肌动蛋白聚合的潜在机制调节乳制品公牛的精子功能和生育能力的睾丸特异性

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摘要

Traditional bull breeding soundness evaluation (BBSE) eliminates bulls that are grossly abnormal; however, bulls classified as satisfactory potential breeders still vary in field fertility, implying submicroscopic differences in sperm characteristics. The testis-specific isoform of Na/K-ATPase (ATP1A4) is involved in regulation of sperm motility and capacitation in bulls through well-established enzyme activity and signaling functions. The objective was to determine ATP1A4 content, activity and their relationship to post-thaw sperm function and field fertility, using semen samples from low-fertility (LF) and high-fertility (HF) Holstein bulls (n = 20 each) with known FERTSOL rates (measure of field fertility, based on non-return rate). Frozen-thawed sperm from HF bulls had increased ATP1A4 content and activity compared to LF bulls. Furthermore, post-thaw sperm from HF bulls had increased tyrosine phosphorylation, ROS, F-actin content, and low intracellular calcium compared to LF bulls. Subsequent incubation of HF bull sperm with ouabain (a specific ligand of Na/K-ATPase) further augmented the post-thaw increase in tyrosine phosphorylation, ROS production, and F-actin content, whereas the increase in intracellular calcium was still low compared to LF bull sperm. ATP1A4 content and activity, ROS, F-actin and calcium were significantly correlated with fertility. In conclusion, we inferred that ATP1A4 content and activity differed among dairy bulls with satisfactory semen characteristics and that ATP1A4 may regulate sperm function through mechanisms involving ROS, F-actin and calcium in frozen-thawed sperm of HF and LF dairy bulls.
机译：传统的公牛育种声音评估（BBSE）消除了严重异常的公牛;然而，分类为令人满意的潜在育种者的公牛仍然因现场生育而变化，暗示精子特征的亚微微镜差异。 Na / K-ATP酶（ATP1A4）的Testis特异性同种型参与通过良好的酶活性和信号功能来调节公牛的精子运动和电容。目的是使用低生育率（LF）和高生育率（HF）Holstein公牛（N = 20）的精液样本来确定ATP1A4含量，活性及其与解冻后精子功能和现场生育能力的含量和现场生育率与已知的Fertsol基于非返回率的率（衡量现场生育率）。与LF公牛相比，HF公牛的冷冻解冻的精子增加了ATP1A4内容和活动。此外，与LF公牛相比，来自HF公牛的解冻精子增加了酪氨酸磷酸化，ROS，F-肌动蛋白含量和低细胞内钙。随后用Ouabain（Na / K-ATP酶的特定配体）孵育HF公牛精子进一步增强了酪氨酸磷酸化，ROS生产和F-肌动蛋白含量的解冻后，而细胞内钙的增加仍然低lf bull sperm。 ATP1A4含量和活性，ROS，F-actin和钙与生育率显着相关。总之，我们推断出ATP1A4内容和活动在乳制品公牛中不同，具有令人满意的精液特征，ATP1A4可以通过涉及ROS，F-Actin和钙的HF和LF乳制品公牛的冷冻解冻精子中的机制来调节精子功能。

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