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首页> 外文期刊>Annals of anatomy =: Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft >Localization pattern of visfatin (NAMPT) in D-galactose induced aged rat testis
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Localization pattern of visfatin (NAMPT) in D-galactose induced aged rat testis

机译：D-半乳糖诱导的老年大鼠睾丸中取磷（Nampt）的定位模式

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摘要

Aging is a complex irreversible process which leads to decline in body physiology including reproductive activity. Neurological and brain functions defects have been studied in the D-gal induced aging rodent model. However, there is dearth of literature on reproductive aging induced by D-gal treatment. Visfatin is an adipokine which regulates testicular steroidogenesis and its level increases under stress conditions to cope and extend longevity. To the best of our knowledge the expression and localization pattern of visfatin and histological evaluaticin of D-gal induced aged testis have not been investigated. Thus, we hypothesized that the expression pattern and histoarchitecture of D-gal induced aged testis are impaired. Therefore, the aim of the present study was to elucidate the histopthological, immunohistochemical localization and expression of visfatin in D-gal induced aged testis along with serum testosterone level, sperm count and daily sperm production. The western blot and immunohistochemical results of the present study showed that D-gal treatment decreases visfatin expression in the testis, particularly in the Leydig cell, and decreases serum testosterone level. Further, D-gal treatment decrease in testosterone levels was positively correlated with decreases in Johnsen's score, mean seminiferous tubule diameter, germinal epithelium height, sperm count and daily sperm production. The multinucleated giant cells showed strong immunostaining for visfatin and suggest the role of visfatin as pro/anti-apoptotic factor. Thus, it can be suggested that visfatin may play an important role in testicular aging by regulating testicular steroidogenesis and spermatogenesis. (C) 2017 Elsevier GmbH. All rights reserved.
机译：老化是一种复杂的不可逆转过程，导致身体生理学的下降，包括生殖活动。 D-GAL诱导老化啮齿动物模型中研究了神经系统和脑功能缺陷。然而，D-GAL治疗诱导的生殖老化存在缺乏文学。游戏素是一种己岛，其调节睾丸类固化性，其水平在应力条件下增加并延长寿命。据我们所知，尚未研究D-GAL诱导的睾丸缺失和组织学评价蛋白的表达和定位模式。因此，我们假设D-GAL诱导的老化睾丸的表达模式和组织建筑受损。因此，本研究的目的是阐明D-GAL诱导的老化睾丸中取酵母的组织学，免疫组织化学定位和表达以及血清睾酮水平，精子计数和每日精子产生。本研究的蛋白质印迹和免疫组织化学结果表明，D-GAL处理降低了睾丸中的缺失表达，特别是在Leydig细胞中，降低血清睾酮水平。此外，D-GAL治疗在睾酮水平下降与约翰森分数的降低，平均血管小管，生发上皮高度，精子计数和每日精子产生呈正相关。多核巨细胞显示出缺霉素的强烈免疫染色，并表明visfatin作为Pro /抗凋亡因子的作用。因此，可以提出取决于通过调节睾丸类固化性和精子发生的睾丸老化在睾丸老化中起重要作用。 （c）2017 Elsevier GmbH。版权所有。

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