首页> 外文期刊>Annals of anatomy =: Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft >Modulation of macrophage phagocytosis in vitro —A role for cholinergic stimulation?
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Modulation of macrophage phagocytosis in vitro —A role for cholinergic stimulation?

机译:调节巨噬细胞吞噬作用在胆碱能刺激的体外作用中的作用?

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Abstract Acetylcholine is synthetized and released from neural cells, but also by non-neuronal cells such as epithelial cells or keratinocytes. Cholinergic agonists enhance the phagocytosis of zymosan particles in primary peritoneal macrophages. The aim of this study was to investigate the effect of carbachol stimulation on phagocytosis in a macrophage cell line using microspheres. The murine cell line MH-S was used in a phagocytosis assay with fluorescent latex beads. The amount of the ingested beads was determined using flow cytometry. Gene expression was investigated using polymerase chain reaction. Gene expression of the muscarinic receptors M1, M3, M4 and M5 but not M2 was found. Carbachol slightly increased the phagocytosis of microspheres in the macrophages. A co-stimulation using lipopolysaccharide and carbachol did not increase the effect of lipopolysaccharide alone. In conclusion, cholinergic stimulation in vitro only moderately modulates the phagocytosis of microspheres. M2 might have a role in stimulation of macrophage phagocytosis. ]]>
机译:摘要乙酰胆碱由神经细胞合成并释放,而且由非神经元细胞如上皮细胞或角蛋白细胞释放。胆碱能激动剂增强原发性腹膜巨噬细胞酵母菌颗粒的吞噬作用。本研究的目的是探讨使用微球的巨噬细胞细胞系中吞噬作用的植物刺激的影响。鼠细胞系MH-S用于用荧光胶乳珠粒吞噬细胞分子测定法。使用流式细胞术测定摄入的珠子的量。使用聚合酶链反应研究基因表达。发现肌肉蛋白受体M1,M3,M4和M5但不是M2的基因表达。卡巴醇略微增加了巨噬细胞中微球的吞噬作用。使用脂多糖和卡巴山的共同刺激并未仅增加脂多糖的作用。总之,体外胆碱能刺激仅适度调节微球的吞噬作用。 M2可能具有刺激巨噬细胞吞噬作用的作用。 ]]>

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