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首页> 外文期刊>Annals of anatomy =: Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft >Advances in cryo-electron tomography for biology and medicine
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Advances in cryo-electron tomography for biology and medicine

机译：生物学和医学的冷冻电子断层扫描进展

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Cryo-electron tomography (CET) utilizes a combination of specimen cryo-fixation and multi-angle electron microscopy imaging to produce three-dimensional (3D) volume reconstructions of native-state macromolecular and subcellular biological structures with nanometer-scale resolution. In recent years, cryo-electron microscopy (cryoEM) has experienced a dramatic increase in the attainable resolution of 3D reconstructions, resulting from technical improvements of electron microscopes, improved detector sensitivity, the implementation of phase plates, automated data acquisition schemes, and improved image reconstruction software and hardware. These developments also greatly increased the usability and applicability of CET as a diagnostic and research tool, which is now enabling structural biologists to determine the structure of proteins in their native cellular environment to sub-nanometer resolution. These recent technical developments have stimulated us to update on our previous review (Koning, R.I., Koster, A.J., 2009. Cryo-electron tomography in biology and medicine. Ann Anat 191, 427-445) in which we described the fundamentals of CET. In this follow-up, we extend this basic description in order to explain the aforementioned recent advances, and describe related 3D techniques that can be applied to the anatomy of biological systems that are relevant for medicine. (C) 2018 Elsevier GmbH. All rights reserved.
机译：冷冻电子断层扫描（CET）利用样品冷冻固定和多角度电子显微镜成像的组合，以产生具有纳米级分辨率的天然状态大分子和亚细胞生物结构的三维（3D）体积重建。近年来，冷冻电子显微镜（CryoEM）经历了可达到的3D重建分辨率的显着增加，这是由电子显微镜技术改进，改善探测器灵敏度，相板的实现，自动数据采集方案和改进的图像的技术改进重建软件和硬件。这些发展也大大提高了CET作为诊断和研究工具的可用性和适用性，现在正在实现结构生物学家，以确定其天然蜂窝环境中蛋白质的结构到亚纳米分辨率。这些最近的技术发展已经促使我们更新我们之前的评论（Koning，R.I.，Koster，A.J.，2009.生物学和医学中的Cryo-Electronicsography。我们描述了CET的基础知识。在这种随访中，我们扩展了这一基本描述，以解释上述最近的进步，并描述了可以应用于与药物相关的生物系统的解剖学的相关3D技术。 （c）2018年Elsevier GmbH。版权所有。

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