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首页> 外文期刊>Annals of anatomy =: Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft >Effect of different resistance-training protocols on the extracellular matrix of the calcaneal tendon of rats
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Effect of different resistance-training protocols on the extracellular matrix of the calcaneal tendon of rats

机译：不同抗性训练方案对大鼠肌腱细胞外基质的影响

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The calcaneal tendon extracellular matrix (ECM) is composed of collagen, non-collagenous glycoproteins and proteoglycans, and able to adapt to various biomechanical stimuli. The objective of this study was to analyze the response of different resistance-training protocols, such as hypertrophy, strength and resistance, on the organization of the calcaneal tendon after training. Wistar rats were divided into four groups: untrained (UT), resistance training (RT), hypertrophy training (HT), and strength training (ST). The protocol in a vertical climbing platform was performed thrice per week over twelve weeks. For biochemical study, the tendons of each group were minced and analyzed for gelatinases, quantification of non-collagenous proteins, sulfated glycosaminoglycans, and hydroxyproline. For morphological analysis, sections were stained with HE and toluidine blue. Non-stained sections were used for birefringence analysis under polarization microscopy. The highest hydroxyproline concentrations were found in HT (154.8 +/- 14.2) and RT (173.6 +/- 25.2) compared with UT (122.4 +/- 27.0). A higher concentration of non-collagenous proteins was detected in the RT group (14.98 mg/g) compared with the other groups. In polarization microscopy, major birefringence was observed in HT and the lowest in ST compared with UT, indicating higher organization of collagen bundles in HT. In analysis for zymography, the presence of latent MMP-9 was more prominent in the ST group and the active MMP-9 more prominent in the HT group. For MMP-2, significant differences in the latent isoform between the HT (184,867 +/- 6765) and UT (173,018 +/- 9696) groups were found. In sections stained with toluidine blue (TB), higher metachromasia was observed in the tendon's distal region in HT and RT groups, indicating a greater amount of proteoglycans. We conclude that the different training protocols produced different responses in the ECM. The remarkable presence of MMP-2 and -9 in the hypertrophy training group may be related to the highest organization of collagen bundles and possibly a more efficient remodeling process, observed in that group, as demonstrated by images and measurements of birefringence. (C) 2017 Elsevier GmbH. All rights reserved.
机译：Calcaneal肌腱细胞外基质（ECM）由胶原，非胶原糖蛋白和蛋白多糖组成，并且能够适应各种生物力学刺激。本研究的目的是分析不同抵抗训练方案的响应，例如培训后对角膜肌腱组织的肥大，力量和抗性。 Wistar大鼠分为四组：未训练（UT），抗性训练（RT），肥大训练（HT）和力量训练（ST）。垂直攀爬平台中的协议每周进行十二周的每周进行三次。对于生化研究，切碎并分析每组的肌腱，并分析凝胶酶，非胶原蛋白的定量，硫酸化糖胺聚糖和羟脯氨酸。对于形态学分析，部分与他和甲苯胺蓝染色。在偏振显微镜下使用未染色的切片进行双折射分析。与UT（122.4 +/- 27.0）相比，在HT（154.8 +/- 14.2）和室温（173.6 +/- 25.2）中发现了最高羟脯氨酸浓度。与其他基团相比，在室温（14.98mg / g）中检测到更高浓度的非胶原蛋白。在偏振显微镜下，与UT相比，在HT和ST中最低的主要双折射，表明HT中的胶原束的更高组织。在分析酶谱系中，在ST组中存在潜伏的MMP-9在HT组中更加突出。对于MMP-2，HT（184,867 +/- 6765）和UT（173,018 +/- 9696）组的潜在同种型的显着差异。在用甲苯胺蓝（TB）染色的部分中，在HT和RT基团的肌腱的远端区域中观察到较高的成阵列，表明较多的蛋白多糖。我们得出结论，不同培训协议在ECM中产生了不同的反应。在肥大训练组中MMP-2和-9的显着存在可能与胶原束的最高组织有关，并且可能在该组中观察到的更有效的重塑过程，如通过图像和双折射的测量所证明的那样。 （c）2017 Elsevier GmbH。版权所有。

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