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首页> 外文期刊>Andrologia >Programmable fast-freezing method improves the post-thaw motion dynamics, integrities of plasmalemma, mitochondrial transmembrane, DNA and, acrosome, and in vivo fertility of water buffalo (Bubalus bubalis) spermatozoa
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Programmable fast-freezing method improves the post-thaw motion dynamics, integrities of plasmalemma, mitochondrial transmembrane, DNA and, acrosome, and in vivo fertility of water buffalo (Bubalus bubalis) spermatozoa

机译：可编程快速冷冻方法改善了解冻后运动动力学，离子血清，线粒体跨膜，DNA和，副骨骼和体内生育率的水牛（血鼠Bubalis）精子

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The effects of freezing methods (FR1, nonprogrammable/static, 5cm above liquid nitrogen [LN2] for 10min, plunging in LN2; FR2, programmable medium, +4 degrees C to -15 degrees C at 3 degrees Cmin(-1), from -15 to -80 degrees C at 10 degrees Cmin(-1) and final holding for 1min at -80 degrees C, plunging in LN2; FR3, programmable fast, from initial holding at +4 degrees C for 2min, from +4 degrees C to -20 degrees C at 10 degrees Cmin(-1), from -20 degrees C to -100 degrees C at 30 degrees Cmin(-1), final holding for 1min at -100 degrees C and plunging in LN2) were assessed on post-thaw in vitro quality and in vivo fertility of water buffalo spermatozoa. Mean sperm progressive motility (%), rapid velocity (%), average path velocity (ms(-1)), straight line velocity (ms(-1)), curved line velocity (ms(-1)), integrities (%) of plasmalemma, mitochondrial transmembrane, DNA and acrosome were higher (p<.05) in samples cryopreserved with FR3 compared to FR1 and FR2. Similarly, in vivo fertility (%) of buffalo spermatozoa was higher (p<.05) with FR3 than FR1 (%; 68.0 versus 50.0). We concluded that programmable fast-freezing method (FR3) improves the post-thaw in vitro quality and in vivo fertility of water buffalo spermatozoa.
机译：冷冻方法（FR1，非可编程/静脉，5cm以上液氮[LN2]的效果10min，在LN2中释放出来，可编程培养基，+ 4℃至-15摄氏度，来自3摄氏度（-1），来自-15至-80°C以10摄氏度（-1）（-1），最终保持1min，在-80℃下，在-80摄氏度下，在LN2中释放出来; FR3，可编程快速，从初始保持在+4摄氏度的初始保持2min，来自+ 4度C至-20摄氏度在10摄氏度（-1），在-20℃至-100摄氏度下，30摄氏度（-1），最终保持在-100摄氏度下1min，并在LN2中释放出来）在水牛精菌水牛体外质量和体内生育率的含铅。平均精子渐进运动（％），快速速度（％），平均路径速度（MS（-1）），直线速度（MS（-1）），曲线速度（MS（-1）），完整性（％ ）与FR1和Fr2相比，用FR3冷冻保存的样品中，线粒体，线粒体跨膜，DNA和取物体较高（P <.05）。类似地，在水牛精子的体内生育率（％）较高（P <.05），FR3比FR1（％; 68.0与50.0）更高（p <.05）。我们得出结论，可编程快速冷冻方法（FR3）改善了水粉中解冻后和水牛精子的生育率。

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