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首页> 外文期刊>Andrologia >Effect of Glyphaea brevis Glyphaea brevis twigs extract on cell viability, apoptosis induction and mitochondrial membrane potential in TM3 Leydig cells
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Effect of Glyphaea brevis Glyphaea brevis twigs extract on cell viability, apoptosis induction and mitochondrial membrane potential in TM3 Leydig cells

机译：Glyphaea Brevis Glyphaea Brevis Twigs提取物在TM3 Leydig细胞中细胞活力，凋亡诱导和线粒体膜电位的影响

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Abstract Glyphaea brevis twigs (Spreng) Monach. (GBT) are used by local herb healers to manage male sexual fertility disorders. The aim of this study was to examine the effects of G.?brevis twigs on TM3 Leydig cells. GBT was extracted using methanol solvent, and Leydig cells were exposed to the respective concentrations of GBT extract (0.1, 1, 10, 100 and 1,000?μg/ml) for 24 and 72?hr respectively. Parameters evaluated include cell morphology, viability (MTT assay), mitochondrial membrane potential (TMRE dye), apoptosis (Annexin V Alexa Fluor 488 binding) and RT‐qPCR analyses of the mRNA expression. Results revealed that GBT had no cytotoxic effect on cell viability and the cell morphology. GBT also revealed a considerable elevation ( p ??0.05) in fluorescence intensity, accompanied by intact mitochondria in TM3 Leydig cells. Furthermore, GBT resulted in the reduction of necrotic and apoptotic cells. The mRNA StAR was upregulated markedly with the effect prominent at 100?μg/ml. This study showed that GBT might be useful for managing male infertility ailments.
机译：抽象glyphaea brevis树枝（Spreng）Monach。 （GBT）由当地的草药治疗师使用来管理男性性肥病。本研究的目的是检查G.®Brevis树枝对TM3 Leydig细胞的影响。使用甲醇溶剂萃取GBT，分别将Leydig细胞暴露于GBT萃取物（0.1,10,100和1,000×μg/ ml）的各自浓度为24和72℃。评估的参数包括细胞形态，活力（MTT测定），线粒体膜电位（TMRE染料），细胞凋亡（annexin v Alexa flul 488结合）和RT-QPCR分析mRNA表达。结果表明，GBT对细胞活力和细胞形态没有细胞毒性作用。 GBT还揭示了荧光强度的相当大的升高（p≤≤0.05），伴随着TM3 Leydig细胞中的完整线粒体。此外，GBT导致坏死和凋亡细胞的减少。 mRNA星状明显上调，在100μg/ ml突出的效果。这项研究表明，GBT可能有助于管理男性不孕症疾病。

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