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Effects of Acremonium terricola culture on performance, milk composition, rumen fermentation and immune functions in dairy cows

机译:临时帝王性Terricola培养对乳奶奶牛性能,牛奶组成,瘤胃发酵和免疫功能的影响

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The effects of Acremonium terricola culture (ATC) on performance, rumen fermentation and microbiota, and antioxidant and immune functions in dairy cows were investigated in two experiments. In Experiment 1, thirty multiparous Holstein dairy cows were blocked for day in milk, somatic cell counts and milk production and were randomly assigned to 1 of 3 treatments in an 8 week trial: a basal diet plus 0, 15 or 30 g/d ATC. The result of supplementing dairy cows with ATC was a linear increase in the apparent digestibility of dry matter, crude protein, acid detergent fiber and neutral detergent fiber, milk production, 4% fat-corrected milk, energy-corrected milk, feed efficiency, milk fat and lactose yields, and milk fat percentage, but a linear decrease in the milk somatic cell count. In Experiment 2, five late-lactating dairy cows were fed the basal diet from d 1 to 14 (pre-trial period). From d 15 to 35 (trial period), the same cows were fed 30 g/d ATC. Supplementation with ATC was stopped from d 36 to 42 (post-trial period). Feed, rumen and blood samples were collected on d 13, 14, 34, 35, 41 and 42. Compared with the pre- and post-periods, the ruminal pH and molar proportion of butyrate decreased during the trial period. Additionally, the ammonia nitrogen and total volatile fatty acid concentrations as well as the molar proportion of acetate were increased. For most of the rumen bacteria that were measured, the relative expression level increased during the trial period, but that of Fibrobacter succinogenes decreased. Moreover, ATC significantly increased the total antioxidant capacity as well as the activities of glutathione peroxidase and total superoxide dismutase during the trial period. The concentrations of blood glucose and immunoglobulins A, M, and G also increased in the plasma. By contrast, ATC resulted in a significant reduction in the plasma concentrations of aspartate transaminase, non-esterified fatty acids, beta-hydroxybutyric acid, interleukin-1, interleukin-6, tumor necrosis factor-alpha and malondialdehyde. These results show that feeding ATC can increase performance, improve rumen fermentation, and increase antioxidant and immune functions in dairy cows, when fed up to 30 g/d.
机译:两次实验研究了仇敌陶氏培养(ATC)对乳制品奶牛的抗氧化和免疫功能的影响。在实验1中,30个多种多种荷斯坦乳制品奶牛在牛奶中封闭,体细胞计数和牛奶生产,并在8周试验中随机分配到3个治疗中的1个:基础饮食加0,15或30g / d ATC 。用ATC补充乳制品奶牛的结果是干物质,粗蛋白,酸性洗涤剂纤维和中性清洁剂,牛奶生产,4%脂肪腐烂的牛奶,能量矫正牛奶,饲料效率,牛奶的表观消化率的线性增加脂肪和乳糖产率,乳脂百分比,但牛奶体细胞计数的线性下降。在实验2中,将五个晚期哺乳酸奶牛从D 1至14(预审期)喂食基础饮食。从D 15到35(试用期),相同的奶牛进给30克/ D ATC。与ATC的补充已从D 36到42(后期后期)停止。收集在D 13,14,32,35,41和42上收集饲料,瘤胃和血液样品。与前期和后期的抑制期相比,试用期期间丁酸丁酸的瘤胃pH和摩尔比例降低。另外,氨氮和总挥发性脂肪酸浓度以及乙酸盐的摩尔比例增加。对于测量的大多数瘤胃细菌,试验期间相对表达水平增加,但纤维杆菌琥珀酰源性降低。此外,ATC在试用期间显着增加了总抗氧化能力以及谷胱甘肽过氧化物酶和总超氧化物歧化酶的活性。血糖和免疫球蛋白A,M和G的浓度也增加了等离子体。相比之下,ATC导致血浆转氨酶的血浆浓度显着降低,非酯化脂肪酸,β-羟基丁酸,白细胞介素-1,白细胞介素-6,肿瘤坏死因子-α和丙二醛。这些结果表明,喂养ATC可以提高性能,提高瘤胃发酵,增加乳制品奶牛的抗氧化剂和免疫功能,当前喂30g / d。

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