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首页> 外文期刊>Annual Review of Analytical Chemistry >Making Sharper Peaks for Reverse-Phase Liquid Chromatography of Proteins
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Making Sharper Peaks for Reverse-Phase Liquid Chromatography of Proteins

机译：制作蛋白质反相液相色谱率的峰值

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摘要

Protein separations have gained increasing interest over the past two decades owing to the dramatic growth of proteins as therapeutics and the completion of the Human Genome Project. About every decade, the field of protein high-performance liquid chromatography (HPLC) seems to mature, having reached what appears to be a theoretical limit. But then scientists well versed in the basic principles of HPLC invented a way around the limit, generating another decade of exciting progress. There is still the need for higher resolution and better compatibility with mass spectrometry because it is an essential tool for identification of proteins and their modifications. To make advances, the fundamental principles need to be understood. This review covers recent advances and current needs in the context of the principles that underlie the many contributions to peak broadening.
机译：由于蛋白质作为治疗剂的显着生长和人类基因组项目的完成，蛋白质分离在过去二十年里越来越多的利益。 关于每十年，蛋白质高效液相色谱（HPLC）的领域似乎成熟，已达到似乎是理论极限。 但随后科学家们熟悉了HPLC的基本原则，发明了一种极限的方式，产生了另一十年的令人兴奋的进步。 仍然需要更高的分辨率和与质谱相容的更好兼容性，因为它是鉴定蛋白质的必要工具及其修改。 为了进展，需要了解基本原则。 本综述涵盖了最近的进展和当前需求，在基本上提出了峰值扩大的许多贡献的背景下。

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