首页> 外文期刊>Annual review of cell and developmental biology >Light-Sheet Microscopy and Its Potential for Understanding Developmental Processes
【24h】

Light-Sheet Microscopy and Its Potential for Understanding Developmental Processes

机译:光纸显微镜及其理解发展过程的潜力

获取原文
获取原文并翻译 | 示例
           

摘要

The ability to visualize and quantitatively measure dynamic biological processes in vivo and at high spatiotemporal resolution is of fundamental importance to experimental investigations in developmental biology.Light-sheet microscopy is particularly well suited to providing such data, since it offers exceptionally high imaging speed and good spatial resolution while minimizing light-induced damage to the specimen.We review core principles and recent advances in light-sheet microscopy, with a focus on concepts and implementations relevant for applications in developmental biology.We discuss how light-sheet microcopy has helped advance our understanding of developmental processes from single-molecule to whole-organism studies, assess the potential for synergies with other state-of-the-art technologies, and introduce methods for computational image and data analysis.Finally, we explore the future trajectory of light-sheet microscopy, discuss key efforts to disseminate new light-sheet technology, and identify exciting opportunities for further advances.
机译:可视化和定量测量体内动态生物方法和高时的能力分辨率的能力对发育生物学的实验研究具有基本的重要性。灯纸显微镜特别适合提供此类数据,因为它提供了非常高的成像速度和良好的成像速度空间分辨率,同时最大限度地减少了对样本的光引起的损坏。我们审查了核心原则和光板显微镜的最新进展,重点是对发展生物学应用相关的概念和实现。我们讨论了光照单层复印件如何帮助推进理解从单分子到全体生物研究的发育过程,评估与其他最先进的技术协同作用的潜力,并引入计算图像和数据分析的方法。最后,我们探讨了光的未来轨迹 - 薄片显微镜,讨论传播新的轻型纸张TEC的关键努力文出,并确定进一步进步的令人兴奋的机会。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号