...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta Physiologiae Plantarum >5-Aminolevulinic acid (ALA) promotes primary root elongation through modulation of auxin transport in Arabidopsis
【24h】

5-Aminolevulinic acid (ALA) promotes primary root elongation through modulation of auxin transport in Arabidopsis

机译：5-氨基乙酰丙烯酸（ALA）通过调节拟南芥的唾液制转运来促进原发性根伸长率

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The specific function of 5-aminolevulinic acid (ALA), a new plant growth regulator, in modulating root growth of plants and the mechanisms underlying ALA-regulated root growth are largely unknown. Here, Arabidopsis seedlings were photographed and collected before and after ALA or 2,3,5-triiodobenzoic acid (TIBA) treatment for determination of root growth, fluorescence intensities of PIN1, PIN2, PIN7, and DR5, and gene expression levels of auxin synthesis, signaling, and transport. We first demonstrated that ALA significantly promoted Arabidopsis primary root elongation. We also found that TIBA, an auxin polar transport inhibitor, inhibited ALA-promoted root elongation, indicating that auxin transport is involved in ALA-regulated root growth. Then, the observations of PIN1, PIN2, and PIN7 at protein and transcript levels suggest that ALA improves auxin transport mainly through regulating auxin efflux carriers. Furthermore, the expression patterns of auxin-responsive reporter DR5rev:GFP were not correlated well with the expression of YUC2, a key biosynthetic gene of auxin, but were consistent with changes of PIN1, PIN2, and PIN7. In addition, ALA did not affect the gene expression of auxin receptor, TRANSPORT-INHIBITOR-RESISTANT1 (TIR1). Taken together, we conclude that ALA promotes primary root elongation of young Arabidopsis seedlings mainly through improving auxin transport. Our data suggest the reciprocal interaction between ALA and auxin, providing new insights into the mechanisms underlying ALA-promoted plant root growth.
机译：5-氨基乙酰乙酸（ALA），一种新的植物生长调节剂，调节植物根生长的特异性和阶段生长的机制在很大程度上是未知的。在此，在ALA或2,3,5-三碘苯苯甲酸（TIBA）处理之前和之后拍摄并收集拟南芥幼苗，用于测定根生长，PIN1，PIN2，PIN7和DR5的荧光强度，以及生长素合成的基因表达水平，信号传播和运输。我们首先展示了ALA显着促进了拟南芥原发性根伸长率。我们还发现TIBA，一种毒素极性传输抑制剂抑制ALA促进的根伸长率，表明养肝转运涉及ALA调节的根生长。然后，蛋白质和转录水平的PIN1，PIN2和PIN7的观察表明ALA主要通过调节养蛋白流出载体来改善疾病传输。此外，随着YUC2的表达，助素响应报告器DR5REV的表达模式与植物素的关键生物合成基因的YUC2的表达不良，但与PIN1，PIN2和PIN7的变化一致。此外，ALA不影响疾病受体的基因表达，抑制抑制剂1（TIR1）。我们得出结论，Ala主要通过改善养羊酸转运来促进年轻拟南芥幼苗的原始根伸长。我们的数据表明ALA和养羊酸之间的互核相互作用，为ALA促进的植物根系生长的机制提供了新的见解。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号