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首页> 外文期刊>Acta Physiologiae Plantarum >Effect of male sterile and fertile cytoplasm on nuclear DNA methylation in hybrid rice
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Effect of male sterile and fertile cytoplasm on nuclear DNA methylation in hybrid rice

机译：雄性不育和肥沃细胞质对杂交水稻核DNA甲基化的影响

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摘要

Nucleus-controlled fertility restoration and cytoplasmic male sterility are important mechanisms to exploit heterosis. However, the effect of DNA methylation on cytoplasmic-nuclear interaction is not well understood yet. The current study used a methylation-sensitive amplified polymorphism to characterize polymorphism in nuclear DNA methylation among cytoplasmic male sterile line (D62A), corresponding maintainer line (D62B), and two F1 hybrids (D62AxR527 and D62BxR527). In results, 495 fragments were amplified between the parental D62A and D62B lines. The total methylation (double+single-stranded) and full methylation (double-stranded) rates of D62A (33.13%, 24.24%) both were found to be lower than that of corresponding maintainer D62B (33.94%, 24.85%). Analysis of methylation revealed that male sterile line D62A was less methylated than that of corresponding maintainer line D62B in all methylation types I, II and III. A total of 516 fragments were amplified between two F1 hybrids (D62AxR527 and D62BxR527). The total methylation in both hybrids (D62AxR527 and D62BxR527) was identical (34.69%). While full methylation rates for D62AxR527 and D62BxR527 were 25.78% and 25.58%, respectively, that is non-significant. Moreover, polymorphism in DNA methylation was found higher in F1 hybrids (5.43%) than parents (4.24%). These results implied that different cytoplasm leads to changes in nuclear DNA methylation and sterile cytoplasm has reduced the effect on nuclear methylation than non-sterile cytoplasm. Current study explains the interaction between cytoplasmic male sterility and DNA methylation which may contribute to further research.
机译：核控制的生育恢复和细胞质雄性不育是利用杂种优势的重要机制。然而，DNA甲基化对细胞质 - 核相互作用的影响尚未得到很好的理解。目前的研究使用了甲基化敏感的扩增多态性，以表征细胞质雄性无菌线（D62A），相应的维护系（D62B）和两个F1杂交种（D62AXR527和D62BXR527）中的核DNA甲基化中的多态性。在结果中，在亲本D62A和D62B线之间扩增495片碎片。发现D62a的总甲基化（双+单链）和全甲基化（双链）速率均低于相应维护器D62b（33.94％，24.85％）。甲基化分析显示，在所有甲基化类型I，II和III中，雄性无菌线D62a小于相应维护系D62b的甲基化。在两个F1杂交物（D62AXR527和D62BXR527之间，共扩增516个片段。杂种（D62AXR527和D62BXR527）中的总甲基化相同（34.69％）。虽然D62AXR527和D62BXR527的全甲基化率分别为25.78％和25.58％，这是非显着的。此外，在F1杂种（5.43％）中发现DNA甲基化中的多态性比父母（4.24％）更高。这些结果暗示不同的细胞质导致核DNA甲基化和无菌细胞质的变化降低了对核甲基化的影响而不是非无菌细胞质。目前的研究解释了细胞质雄性不育与DNA甲基化之间的相互作用，这可能有助于进一步研究。

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  • 来源
    《Acta Physiologiae Plantarum》 |2019年第6期|共7页
  • 作者

    Ali Asif; Li Yun; Chen Hui; Xu Peizhou; Zhang Hongyu; Chen Xiaoqiong; Liao Yongxiang; Fu Shaohong; Wu Tingkai; Iqbal Muhammad Zafar; Farooq Muhammad Umer; Wu Xianjun;

  • 作者单位

    Sichuan Agr Univ Minist Educ Key Lab Southwest Crop Genet Resources &

    Genet Im Rice Res Inst Chengdu Sichuan Peoples R China;

    Chengdu Acad Agr &

    Forestry Sci Chengdu Sichuan Peoples R China;

    Sichuan Agr Univ Minist Educ Key Lab Southwest Crop Genet Resources &

    Genet Im Rice Res Inst Chengdu Sichuan Peoples R China;

    Sichuan Agr Univ Minist Educ Key Lab Southwest Crop Genet Resources &

    Genet Im Rice Res Inst Chengdu Sichuan Peoples R China;

    Sichuan Agr Univ Minist Educ Key Lab Southwest Crop Genet Resources &

    Genet Im Rice Res Inst Chengdu Sichuan Peoples R China;

    Sichuan Agr Univ Minist Educ Key Lab Southwest Crop Genet Resources &

    Genet Im Rice Res Inst Chengdu Sichuan Peoples R China;

    Sichuan Agr Univ Minist Educ Key Lab Southwest Crop Genet Resources &

    Genet Im Rice Res Inst Chengdu Sichuan Peoples R China;

    Chengdu Acad Agr &

    Forestry Sci Chengdu Sichuan Peoples R China;

    Sichuan Agr Univ Minist Educ Key Lab Southwest Crop Genet Resources &

    Genet Im Rice Res Inst Chengdu Sichuan Peoples R China;

    Sichuan Agr Univ Maize Res Inst Chengdu Sichuan Peoples R China;

    Sichuan Agr Univ Minist Educ Key Lab Southwest Crop Genet Resources &

    Genet Im Rice Res Inst Chengdu Sichuan Peoples R China;

    Sichuan Agr Univ Minist Educ Key Lab Southwest Crop Genet Resources &

    Genet Im Rice Res Inst Chengdu Sichuan Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物学;
  • 关键词

    CMS; F1 hybrid; Methylation level; MSAP; Polymorphism;

    机译：CMS;F1杂交;甲基化水平;MSAP;多态性;

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