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首页> 外文期刊>American Journal of Sports Medicine >Are Amniotic Fluid Products Stem Cell Therapies? A Study of Amniotic Fluid Preparations for Mesenchymal Stem Cells With Bone Marrow Comparison
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Are Amniotic Fluid Products Stem Cell Therapies? A Study of Amniotic Fluid Preparations for Mesenchymal Stem Cells With Bone Marrow Comparison

机译：是羊水产品干细胞疗法吗？ 具有骨髓比较的间充质干细胞羊水制剂研究

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Background: In vivo amniotic fluid is known to contain a population of mesenchymal stem cells (MSCs) and growth factors and has been shown to assist in healing when used as an adjunct in procedures across multiple medical specialties. It is unclear whether amniotic fluid products (AFPs) contain MSCs and, if so, whether the cells remain viable after processing. Purpose: To determine whether MSCs, growth factors, and hyaluronan are present in commercially available AFPs. Study Design: Descriptive laboratory study. Methods: Seven commercial companies that provide amniotic fluid were invited to participate in the study; 3 companies (the manufacturers of PalinGen, FloGraft, and Genesis AFPs) agreed to participate and donated AFPs for analysis. The AFPs were evaluated for the presence of MSCs, various growth factors relevant to orthopaedics (platelet-derived growth factor ββ, vascular endothelial growth factor, interleukin 8, bone morphogenetic protein 2, transforming growth factor β_(1)), and hyaluronan by enzyme-linked immunosorbent assay and culture of fibroblast colony-forming units. These products were compared with unprocessed amniotic fluid and 2 separate samples of MSCs derived from human bone marrow aspirates. All groups used the same culture medium and expansion techniques. Identical testing and analysis procedures were used for all samples. Results: MSCs could not be identified in the commercial AFPs or the unprocessed amniotic fluid. MSCs could be cultured from the bone marrow aspirates. Nucleated cells were found in 2 products (PalinGen and FloGraft), but most of these cells were dead. The few living cells did not exhibit established characteristics of MSCs. Growth factors and hyaluronan were present in all groups at varying levels. Conclusion: The AFPs studied should not be considered “stem cell” therapies, and researchers should use caution when evaluating commercial claims that products contain stem cells. Given their growth factor content, however, AFPs may still represent a promising tool for orthopaedic treatment. Clinical Relevance: Amniotic fluid has been proposed as an allogenic means for introducing MSCs. This study was unable to confirm that commercial AFPs contain MSCs.
机译：背景：已知在体内羊水中含有间充质干细胞（MSCs）和生长因子的群体，并且已被证明当用作多种医学专业的程序中的辅助程序时有助于愈合。目前尚不清楚羊水产物（AFP）是否含有MSC，如果是，该细胞在处理后是否保持可行性。目的：确定MSC，生长因子和透明质酸是否存在于商业上的AFP中。研究设计：描述性实验室研究。方法：邀请七家提供羊水的商业公司参加该研究; 3家公司（普拉明，浮虫和创世纪的制造商）同意参加和捐赠AFP进行分析。评估AFPS的存在MSCs，与骨科相关的各种生长因子（血小板衍生的生长因子ββ，血管内皮生长因子，白细胞介素8，骨质发生蛋白2，转化生长因子β_（1））和羟基尼-Linded的免疫吸附测定和成纤维细胞菌落形成单元的培养。将这些产物与未加工的羊水和2种不同的MSC样品进行比较。衍生自人骨髓抽吸物。所有团体都使用相同的培养基和扩展技术。所有样品使用相同的测试和分析程序。结果：无法在商业组织或未加工的羊水中识别MSCs。 MSCs可以从骨髓吸食中培养。在2种产品（普利语和浮法移植物）中发现了核细胞，但大多数这些细胞死了。少数生命细胞没有表现出MSCs的既定特征。在不同水平的所有群体中存在生长因子和透明质酸。结论：研究中的法语不应被视为“干细胞”疗法，研究人员应在评估产品含有干细胞时小心。然而，鉴于他们的生长因子含量，AFP仍然可能代表骨科治疗的有希望的工具。临床相关性：已提出羊水作为引入MSCs的同种异体手段。本研究无法确认商业AFP包含MSC。

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