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首页> 外文期刊>Applied Biological Chemistry >Cloning of an organophosphorus hydrolase (opdD) gene of Lactobacillus sakei WCP904 isolated from chlorpyrifos-impregnated kimchi and hydrolysis activities of its gene product for organophosphorus pesticides
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Cloning of an organophosphorus hydrolase (opdD) gene of Lactobacillus sakei WCP904 isolated from chlorpyrifos-impregnated kimchi and hydrolysis activities of its gene product for organophosphorus pesticides

机译：从氯吡啶浸渍的Kimchi中分离出乳酸杆菌的有机磷酸盐水解酶（OPDD）基因的克隆，其基因产物的有机磷农药的水解活性

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摘要

Chlorpyrifos (CP) residues are absorbed from soil and often found in Korean cabbages that are being used to make kimchi. Lactobacillus sakei WCP904, harboring the organophosphorus (OP) hydrolase gene opdD, was isolated from CP-impregnated mulkimchi. The cloned gene opdD from strain CP904 comprises 825 base-pair nucleotides that encode 274 amino acids. The recombinant Escherichia coli harboring the opdD gene depleted 73% of CP after 6days in M9 medium. In fact, the OpdD protein is a novel member of the GHSQG family of esterolytic enzymes or lactic acid bacterial Opd groups. The molecular weight of the OpdD protein was estimated to be 31kDa using SDS-PAGE. Broad-spectrum activities of the OpdD protein were obtained against OP insecticides containing both P-O and P-S bonds. The OpdD protein exhibits maximum activity at 30 degrees C with pH 6. No enzyme activities of the mutated OpdD (Ser116Ala116) protein toward -nitrophenyl butyrate and CP substrates were observed. These results suggested that the strain WCP904 scavenges insecticide residues from mulkimchi vegetables, thus abolishing health hazards by secreting OP hydrolase during fermentation.
机译：氯吡啶（CP）残留物由土壤吸收，经常在韩国型腹部发现，用于制作泡菜。含有有机磷（OP）水解基因OPDD的Lactobacillus Sakei WCP904与CP浸渍的Mulkimchi分离。来自菌株CP904的克隆基因OPDD包含825个碱基对核苷酸，其编码274个氨基酸。在M9培养基中，重组大肠杆菌含有OPDD基因的CP耗尽73％CP。事实上，OPDD蛋白是GHSQG家族的新型酯类酶或乳酸菌OPD组的新成员。使用SDS-PAGE估计OPDD蛋白的分子量为31KDA。含有含有P-O和P-S键的OPDD蛋白的广谱活性。 OPDD蛋白在30℃下具有pH6的最大活性。观察到突变的OPDD（Ser116Ala116）蛋白的酶活性偏向 - 硝基苯丁酸酯和CP底物。这些结果表明，菌株WCP904清除了来自Mulkimchi蔬菜的杀虫剂残留物，从而通过在发酵过程中分泌op水解酶来消除健康危害。

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