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首页> 外文期刊>Antonie van Leeuwenhoek: Journal of Microbiology and serology >Paracoccus pueri sp nov., isolated from Pu'er tea
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Paracoccus pueri sp nov., isolated from Pu'er tea

机译：Paracoccus儿童sp。，从普洱茶隔离

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摘要

A Gram-stain negative, aerobic, short rod-shaped, motile by flagella bacterial strain (THG-N2.35(T)), was isolated from Pu'er tea. Growth occurred at 10-40 A degrees C (optimum 28 A degrees C), at pH 4-7 (optimum 7) and at 0-5% NaCl (optimum 1%). Based on 16S rRNA gene sequence analysis, the near phylogenetic neighbours of strain THG-N2.35(T) were identified as Paracoccus hibisci KACC 18632(T) (99.0%), Paracoccus tibetensis CGMCC 1.8925(T) (98.7%), Paracoccus beibuensis CGMCC 1.7295(T) (98.2%), Paracoccus aestuarii KCTC 22049(T) (98.2%), Paracoccus rhizosphaerae LMG 26205(T) (98.1%), Paracoccus zeaxanthinifaciens ATCC 21588(T) (97.1%), Paracoccus marcusii DSM 11574(T) (97.0%). Levels of similarity between strain THG-N2.35(T) and other Paracoccus species were lower than 97.0%. DNA-DNA hybridization values between strain THG-N2.35(T) and P. hibisci KACC 18632(T), P. tibetensis CGMCC 1.8925(T), P. beibuensis CGMCC 1.7295(T), P. aestuarii KCTC 22049(T), P. rhizosphaerae LMG 26205(T), P. zeaxanthinifaciens ATCC 21588(T), P.marcusii DSM 11574(T) were 47.5% (42.3%, reciprocal analysis), 36.1% (32.3%), 24.7% (22.1%), 19.2% (16.3%), 11.3% (8.8%), 11.1% (10.8%), 6.1% (5.8%), respectively. The DNA G+C content of strain THG-N2.35(T) was 62.3 mol%. The polar lipids were diphosphatidylglycerol, phosphatidyl-N-methylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The quinone was ubiquinone-10 (Q-10). The major fatty acids were C-10:0 3OH, C-16:0, C-18:0 and C-18:1 omega 7I(2). On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-N2.35(T) represent a novel species of the genus Paracoccus, for which the name Paracoccus pueri sp. nov. is proposed. The type strain is THG-N2.35(T) (= KACC 18934(T) = CCTCC AB 2016177(T)).
机译：葡萄污染剂负数，有氧，短杆状，由壁炉菌菌株（THG-N2.35（T））的动机从普洱茶中分离。生长发生在10-40℃（最佳28℃），pH 4-7（最佳7）和0-5％NaCl（最佳1％）。基于16S rRNA基因序列分析，菌株THG-N2.35（T）的近弦发育邻居被鉴定为帕拉科治术术18632（T）（99.0％），帕拉科治肝菌菌菌，肝癌（98.7％）（98.7％），Paracoccus Beibuensis CGMCC 1.7295（T）（98.2％），裂藻球菌Aestuarii KCTC 22049（T）（98.2％），裂藻球菌Rhizosphaerae LMG 26205（T）（98.1％），裂藻球菌Zeax分子嘧啶ATCC 21588（T）（97.1％），帕拉科克马库斯马氏菌DSM 11574（T）（97.0％）。菌株THG-N2.35（T）与其他裂开气体物种之间的相似性水平低于97.0％。菌株THG-N2.35（T）和P. hibisci KACC 18632（T），P.Ibetensis CGMCC 1.8925（T），P.Beibuensis CGMCC 1.7295（T），P.Asestuarii KCTC 22049（T）之间的DNA-in ），P.Rhizosphaerae LMG 26205（T），P.ZeAxanthinifaciens ATCC 21588（T），P.Marcusii DSM 11574（T）为47.5％（42.3％，互核分析），36.1％（32.3％），24.7％（22.1 ％），19.2％（16.3％），11.3％（8.8％），11.1％（10.8％），6.1％（5.8％）。菌株THG-N2.35（T）的DNA G + C含量为62.3mol％。极性脂质是二磷脂酰甘油，磷脂酰-N-甲乙醇胺，磷脂酰乙醇胺，磷脂酰甘油和磷脂酰胆碱。醌是泛醌-10（Q-10）。主要脂肪酸是C-10：0 3 OH，C-16：0，C-18：0和C-18：1 Omega 7i（2）。在系统发育分析的基础上，趋化性数据，生理特性和DNA-DNA杂交数据，菌株THG-N2.35（T）代表了帕拉科克属的新种类，名称Paracoccus Pueri Sp。 11月。提出。类型应变是THG-N2.35（T）（= KACC 18934（T）= CCTCC AB 2016177（T））。

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