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首页> 外文期刊>Applied immunohistochemistry and molecular morphology: AIMM >Comparison of beta-Catenin and LEF1 Immunohistochemical Stains in Desmoid-type Fibromatosis and its Selected Mimickers, With Unexpected Finding of LEF1 Positivity in Scars
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Comparison of beta-Catenin and LEF1 Immunohistochemical Stains in Desmoid-type Fibromatosis and its Selected Mimickers, With Unexpected Finding of LEF1 Positivity in Scars

机译：β-catenin和Lef1免疫组织化学污渍在Demoid-型纤维瘤病和其所选模拟器中的比较，意外发现伤疤中的lef1阳性

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摘要

P-catenin immunohistochemical stain can be useful in the diagnosis of many tumors including desmoid-type fibromatosis (DTF). Lymphoid enhancer-factor 1 (LEF1), a recently emerged marker, is part of the Wnt pathway with P-catenin but has not been studied in DTF. We performed LEF1 and P-catenin immunohistochemistry in DTF (n = 26), superficial fibromatosis (n = 19), sclerosing mesenteritis (n = 12), gastrointestinal stromal tumor (n = 17), and cutaneous scar (n = 14) using tissue microarray and whole sections. The staining intensity was scored as strong (visible at x 2 objective, value of 3), moderate (visible at x 4, value of 2), weak (visible at x 10, value of 1), and negative (not visible at x 10, value of 0). The percentage of positive nuclei was recorded in 10% increment. Histologic scores were generated by multiplying numerical value of intensity and percentage of positive nuclei. A score of at least 10 was defined as positive. Eighteen of the 25 DTF were positive for LEF1 while 12 of 25 were positive for P-catenin (1 excluded due to loss of tissue). Gastrointestinal stromal tumor cases were negative for both markers. All superficial fibromatoses were negative except 2 cases with weak positivity for LEF1 but not P-catenin. Only 2 case of sclerosing mesenteritis were weakly positive for LEF1 but negative for P-catenin. Ten of 14 scars were positive for LEF1 but only 1 of them was weakly positive for p-catenin. In conclusion, this study demonstrated that LEF1 may be a useful marker in the differential diagnosis of DTF in certain contexts. However, caution should be exercised since LEF1 positivity can also be seen in scars.
机译：P-Catenin免疫组化染色剂可用于诊断许多肿瘤，包括Demopoid型纤维瘤病（DTF）。淋巴增强剂因子1（LEF1）是最近出现的标志物，是WNT途径的一部分，但尚未在DTF中进行研究。我们在DTF（n = 26）中进行了lef1和p-catenin免疫组化学，浅表纤维瘤症（n = 19），硬化肠系膜炎（n = 12），胃肠道间质瘤（n = 17），和皮肤疤痕（n = 14）使用组织微阵列和整个部分。染色强度得分强度（在x 2目标，值为3），中等（在x 4，值为2），弱（在x 10，值1的值下可见），负（在x 10，值下） 10，值0）。阳性核的百分比以10％的增量记录。通过乘以阳性核的强度和百分比的数值来产生组织学分数。得分至少为10的定义为正。 25dTF的18个对于lef1是阳性的，而第12个中的12个阳性对于p-catenin（1由于组织损失而排除）。两种标记的胃肠道肿瘤病例为阴性。除了lef1弱阳性外，所有浅表纤维菌均为阴性，除了p-catenin弱。只有2例硬化的肠系膜炎对lef1弱阳性，但对p-catenin负阴性。十分之一的伤疤对于lef1是阳性的，但只有1只对p-catenin弱阳性。总之，本研究表明，在某些情况下，LEF1可以是DTF的差异诊断中的有用标志物。但是，应该注意，由于lef1阳性也可以在疤痕中看到。

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