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首页> 外文期刊>Aquaculture International >Identification of giant gourami iridovirus (GGIV): a new infectious spleen and kidney necrosis virus (ISKNV) from natural outbreak in cultured Osphronemus goramy
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Identification of giant gourami iridovirus (GGIV): a new infectious spleen and kidney necrosis virus (ISKNV) from natural outbreak in cultured Osphronemus goramy

机译：鉴定巨痛伊米莫西病毒（GGIV）：从培养的奥萨克脲属植物中自然爆发的新传染性脾脏和肾脏坏死病毒（Isknv）

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Megalocytivirus of family Iridoviridae has been identified as a pathogen that caused the fatal systemic infection to lead to massive death of numerous fish species in both ornamental and food fish industries. It caused significant economic losses due to severe mortality of infected fish. Since 2011 in Indonesia, megalocytivirus in giant gourami (Osphronemus goramy) had been found as the main pathogen in some cases of giant gourami outbreak, especially in West Java, Central Java, and Bali. The aim of this study is to identify and characterize the pathogen known as a megalocytivirus infection in freshwater-cultured giant gourami. We identified the virus using universal and specific primers for megalocytivirus and infectious spleen and kidney necrosis virus (ISKNV). Sequencing and BLAST analysis were used to develop a phylogenetic tree. The result showed that phylogenetic analysis of major capsid protein (MCP) gene unveiled a new member of megalocytivirus, designated as giant gourami iridovirus (GGIV). GGIV-formed cluster belonged to ISKNV and has 100% homology to ISKNV complete genome. Artificial infection by intraperitoneal injection with supernatant homogenate from spleen and kidney of naturally infected fish showed 93% cumulative mortality in 12 days. Fish showed a clinical sign of infection as lethargic, loss of appetite, pale or darken body color, and hemorrhages. Internal organ on dead fish showed swollen spleen and kidney and also a pale liver. Quantitative PCR analysis on internal organs showed spleen had the highest viral DNA copy number followed by kidney, gill, and liver. Histopathological analysis showed many abnormally hypertrophied cells in spleen which is typical histopathological characteristic of megalocytivirus infection. In conclusion, GGIV belonged to ISKNV from genus megalocytivirus.
机译：伊米多维尿的巨大炎已被鉴定为一种病原体，导致致命的全身感染导致观赏和食品鱼类工业中许多鱼类的大规模死亡。由于受感染鱼的严重死亡率，它引起了显着的经济损失。自2011年以来，在巨型Gourami（奥氨胺属oborams）中的巨粒细胞病毒在一些巨大的Gourami爆发中被发现是主要的病原体，特别是在西爪哇省，中爪哇省和巴厘岛。本研究的目的是鉴定和表征淡水培养的巨幼瓜中称为Megalocytivirus感染的病原体。我们用巨大和特异性引物鉴定了巨胶和传染性脾脏和肾脏坏死病毒（Isknv）的病毒。使用测序和爆炸分析来开发系统发育树。结果表明，主要衣壳蛋白（MCP）基因的系统发育分析揭开了巨大的葡萄球菌的新成员，被称为巨大的甘氨酸Iridovirus（GGIV）。 GGIV成立的群体属于IsknV，并具有100％同源性对IsknV完全基因组。通过腹膜内注射的人工感染与自然感染的鱼的脾脏和肾脏上清液匀浆，在12天内显示出93％的累积死亡率。鱼展示了感染的临床迹象，作为昏昏欲睡，食欲丧失，苍白或黑暗的身体颜色，和出血。死鱼内器官显示脾脏和肾脏肿胀，也是苍白的肝脏。内脏的定量PCR分析显示脾脏具有最高的病毒DNA拷贝数，然后是肾，鳃和肝脏。组织病理学分析表明，脾脏中许多异常的肥大细胞，这是巨大细胞感染的典型组织病理学特征。总之，GGIV属于Megalocytivirus属的Isknv。

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