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首页> 外文期刊>Aquaculture International >High mortalities caused by Aeromonas veronii:identification, pathogenicity, and histopathologicalstudies in Oreochromis niloticus
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High mortalities caused by Aeromonas veronii:identification, pathogenicity, and histopathologicalstudies in Oreochromis niloticus

机译：由Aeromonas Veronii引起的高死亡率：厄勒莫里氏菌中的鉴定，致病性和组织病理学术

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摘要

The current study planned to identify suspected causes of mass mortalities in Oreochromis niloticus at private fish farms in Port Said Governorate, Egypt, during the 2017 summer season. One hundred twenty diseased moribund O. niloticus fish were randomly collected from different locations. The examined fish showed lethargy, dark skin, bilateral exophthalmia with corneal opacity, and varying degrees of ulcers. Internal examination revealed generalized inflammation, congestion, hemorrhaging, and enlargement of most internal organs. Identification was accomplished through analytical profile index (API) 20E and polymerase chain reaction (PCR) and confirmed by sequencing and phylogenetic analysis. An antibiogram was constructed based on results from a disc diffusion test. Experimental infection was also performed and showed 80% mortality. Virulence genes were also assayed. Histopathological examination of experimentally challenged fish revealed prominent pathological lesions (mainly necrosis), degenerative changes, and cellular vacuolation. In conclusion, Aeromonas veronii can be considered one of the main causes of summer mass mortalities in O. niloticus in the study area.
机译：目前的研究计划在2017年夏季期间，识别港口港口私营农场的禁止欧洲菌群大规模死亡原因。一百二十次患病摩尔德·诺伊洛鱼鱼被随机从不同的地方收集。检查的鱼显示嗜睡，深色皮肤，双侧细胞凋亡，角膜不透明度，溃疡变化。内部检查揭示了大多数内器官的广义炎症，充血，出血和扩大。通过分析轮廓指数（API）20E和聚合酶链反应（PCR）来完成鉴定并通过测序和系统发育分析证实。基于盘扩散试验的结果构建抗诊断。还进行了实验性感染并显示出80％的死亡率。毒力基因也被测定。实验挑战性鱼的组织病理学检查显示出突出的病理病变（主要是坏死），退行性变化和细胞真空。总之，Aeromonas Veronii可以被认为是研究区域O. Niloticus夏季大众凡人的主要原因之一。

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