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首页> 外文期刊>Aquaculture International >The feasibility of using primary shrimp hemocyte culture to screen herbal immunostimulants
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The feasibility of using primary shrimp hemocyte culture to screen herbal immunostimulants

机译：使用虾血细胞培养物筛选草药免疫刺激剂的可行性

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摘要

An analysis of the immune activity of herbal extract in vivo and in vitro assays was performed to explore the feasibility of using primary shrimp hemocyte culture to screen herbal immunostimulants. In vitro experiment was conducted by adding herbal extract into the medium of primary cultured hemocytes and incubating for 24 h. In vivo experiment was carried out by feeding shrimp with diets incorporated with herbal extract for 21 days. Challenge test can be used to directly reflect comprehensive immune capacity of organisms. Four immune indexes, total hemocyte counts (THC and lysozyme, phenoloxidase (PO), and phagocytic activity were examined both in vitro and in vivo. The results showed that effects of herbal extract on THC or lysozyme activity were different between in vitro and in vivo. On the contrary, effects of herbal extract on PO activity and phagocytic activity in vitro and in vivo showed a strong correlation. Herbal extract, which enhanced PO activity and phagocytic activity in vitro, had ultimate immune-promoting function in vivo. Therefore, it was feasible to use primary hemocyte culture to screen herbal immunostimulants by determining the effects of herbal immunostimulants on PO and phagocytic activities.
机译：进行体内和体外测定中草药提取物的免疫活性分析，以探讨使用原代虾血细胞培养到筛选草药免疫刺激剂的可行性。通过将草药提取物添加到原发性培养的血细胞培养基中并孵育24小时来进行体外实验。在体内实验中，通过将虾与中草提取物掺入21天的饮食中进行。挑战试验可用于直接反映生物体的综合免疫能力。在体外和体内检查四种免疫指标，总血液细胞计数（THC和溶菌酶，酚氧基酶（PO）和吞噬活性。结果表明，在体外和体内，草药提取物对THC或溶菌酶活性的影响。相反，草药提取物对体外和体内Po活性和吞噬活性的影响表现出强烈的相关性。中草提取物，增强Po活性和体外吞噬活性的植物提取物在体内具有最终免疫促进功能。因此通过确定草药免疫刺激剂对PO和吞噬活性的影响，使用初级血细胞培养物来筛选草药免疫刺激剂是可行的。

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