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首页> 外文期刊>Aquaculture International >Sperm vitrification of Litopenaeus vannamei: effect of cryoprotectant solutions on sperm viability and spawning quality after artificial insemination
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Sperm vitrification of Litopenaeus vannamei: effect of cryoprotectant solutions on sperm viability and spawning quality after artificial insemination

机译：Litopenaeus Vannamei的精子玻璃化：冷冻保护剂对人工授精后的精子活力和产卵质量的影响

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摘要

Vitrification is a fast freezing method with promising results for penaeids sperm cryopreservation. This study evaluated the efficiency of three cryoprotectant solutions for sperm vitrification and artificial insemination with cryopreserved spermatophores of Litopenaeus vannamei. The cryoprotectant solutions tested were 30% methanol, 2% soy lecithin, and 30% methanol + 2% soy lecithin. Fully mature females were artificially inseminated with vitrified and fresh spermatophores as a control group. The vitrification method was efficient in maintaining high rates of sperm survival and membrane integrity. Although the egg fertilization was successfully attained by artificial insemination with cryopreserved spermatophores, low hatching rates suggested that possible DNA fragmentation of sperm cells should be further investigated. This is the first report of artificial insemination using vitrified sperm in penaeids.
机译：玻璃化是一种快速冷冻方法，具有对Penaeids精子冷冻保存的有前途的结果。 本研究评估了三种冷冻保护剂的精子玻璃化和人工授精的效率与Litopenaeus Vannamei的冷冻保存精菌。 测试的冷冻保护溶液是30％甲醇，2％大豆卵磷脂和30％甲醇+ 2％大豆卵磷脂。 完全成熟的女性人为植入玻璃化和新鲜精子作为对照组。 玻璃化方法有效地保持了精子存活率的高速率和膜完整性。 尽管通过用冷冻保存的精子成功地获得了蛋精，但低孵化率表明，应进一步研究可进一步研究可切割精子细胞的DNA片段化。 这是使用Penaeids中使用玻璃化精子的人工授精的第一报告。

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