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首页> 外文期刊>Aquaculture International >Cryopreservation of sperm in brown-marbled grouper (Epinephelus fuscoguttatus)
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Cryopreservation of sperm in brown-marbled grouper (Epinephelus fuscoguttatus)

机译：棕色大理石疱疹的精子冷冻保存（Epinephelus fuscoguttatus）

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摘要

The brown-marbled grouper, Epinephelus fuscoguttatus, is a highly commercial fishery and mariculture species listed as Near Threatened by IUCN. Adequate supply of good quality seed, at present, has become a bottleneck for grouper farming due to the rapid expansion of aquaculture practices. As sperm cryopreservation is of great potential not only for fish farming but also for species conservation, a series of sequential experiments were carried out to determine optimum cryoprotectant, freezing condition, dilution ratio, equilibration time, and thawing temperature for freezing brown-marbled grouper sperm. Based on post-thaw motility, 10% DMSO combined with 0.3 M glucose shown to be an effective cryomedium among the cryoprotectants evaluated here, and sperm was better cryopreserved by freezing 1-5 cm above the liquid nitrogen (LN) surface and cooled below - 80 degrees C before plunging into LN. Significantly higher post-thaw motility was observed for dilution ratios ranging from 4:1 to 1:3 than at higher dilution ratios (1:5, 1:9, and 1:19). Besides, equilibrated and post-thaw sperm motility was not affected by a 2-h equilibration period, indicating a high level of toxicity tolerance for 10% DMSO. Further evaluation of thawing temperatures showed that 30 degrees C and 40 degrees C yielded significantly higher post-thaw motility than at 25 degrees C, 50 degrees C, or 60 degrees C. In addition, brown-marbled grouper sperm could be refrigerated up to 48 h without decreasing its suitability for cryopreservation. Compared with fresh sperm, a decrease in motility and curvilinear velocity (VCL) was detected in thawed sperm. However, the thawed sperm could be stored up to 2 h at 4 degrees C without losing its fertilizing capacity, which was equal to that of fresh sperm (fertilization rate, 92.7 +/- 2.5%; hatching rate, 87.7 +/- 2.1%). The cryopreservation protocol developed in this study is efficient and will facilitate seed production for both aquaculture and conservation purposes.
机译：棕色大理石疱疹石斑鱼，Epinephelus fuscoguttatus是一家高度商业的渔业和海水养殖物种，被IUCN受到威胁。目前已充分供应优质种子，由于水产养殖实践的快速扩张，由于水产养殖实践的快速扩张，已成为石斑鱼种植的瓶颈。由于精子冷冻保存不仅适用于鱼类养殖，而且对于物种保护，还进行了一系列顺序实验，以确定冷冻棕色大理石木质石斑鱼精子的最佳冷冻保护剂，冷冻条件，稀释比，平衡时间和解冻温度。基于解冻后运动，10％DMSO与0.3M葡萄糖相结合，在此评价的冷冻保护剂中显示为有效的漏洞，并且精子通过冷冻1-5cm在液氮（LN）表面上方并冷却下方 - 在进入LN之前80度C.观察到从4：1至1：3的稀释比率观察到显着提高的解冻后动力比在更高的稀释比（1：5,1：9和1:19）中的稀释比率。此外，平衡和后解冻精子运动不受2-H平衡周期的影响，表明10％DMSO的高水平毒性耐受性。进一步评估解冻温度的评价显示，30摄氏度和40℃的后解冻动力显着高于25℃，50℃或60℃。此外，棕色大理石纹装精子可冷藏高达48 H不降低其对冷冻保存的适用性。与新鲜精子相比，在解冻的精子中检测到动力和曲线速度（VCl）的降低。然而，将解冻的精子在4摄氏度下储存高达2小时，而不会使其施肥能力等于新鲜精子（施肥率，92.7 +/- 2.5％;孵化率，87.7 +/- 2.1％ ）。本研究开发的冷冻保存协议是有效的，将促进种子生产，以便为水产养殖和保护目的进行播种。

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