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首页> 外文期刊>Aquaculture Nutrition >Dietary n-3 LC-PUFAs affect lipoprotein lipase (LPL) and fatty acid synthase (FAS) activities and mRNA expression during vitellogenesis and ovarian fatty acid composition of female silver pomfret (Pampus argenteus) broodstock
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Dietary n-3 LC-PUFAs affect lipoprotein lipase (LPL) and fatty acid synthase (FAS) activities and mRNA expression during vitellogenesis and ovarian fatty acid composition of female silver pomfret (Pampus argenteus) broodstock

机译:膳食N-3 LC-PUFA影响脂蛋白脂肪酶(LPL)和脂肪酸合成酶(FAS)活性和MRNA表达在雌性银粉(Pampus Argenteus)Broodstock的vitellogisesis和卵巢脂肪酸组成中

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摘要

We studied the effects of dietary n-3 LC-PUFAs on the activities and mRNA expression levels of tissue lipoprotein lipase (LPL) and fatty acid synthase (FAS) during vitellogenesis and ovarian fatty acid composition in female silver pomfret broodstock. Broodstock were fed one of four experimental diets for 185 days: FO (100% fish oil), FSO (70% fish oil + 30% soybean oil), SFO (30% fish oil + 70% soybean oil) or SO (100% soybean oil). The results revealed that hepatic LPL and FAS and ovarian FAS activities and mRNA expression levels significantly increased at vitellogenesis and postvitellogenesis relative to previtellogenesis, with no significant differences between these two stages, except for hepatic LPL mRNA expression. Dietary n-3 LC-PUFAs decreased tissue FAS and increased LPL activities and mRNA expression levels. The ovarian concentrations of 20:4n-6 (ARA), 20:5n-3 (EPA), 22:6n-3 (DHA) and n-3 LC-PUFAs were directly influenced by n-3 LC-PUFA levels. Total n-3 LC-PUFA concentrations in SO were 57% lower than those in FO, while 18:2n-6 concentrations in SO were 4.7 x higher than those in FO. These results revealed that high dietary n-3 LC-PUFAs levels significantly affected tissue lipid metabolism in female silver pomfret broodstock during vitellogenesis by upregulating LPL and downregulating FAS.
机译:我们研究了饮食N-3 LC-PUFA对女性银POMFRet中的培体和卵巢脂肪酸组合物的组织脂蛋白脂肪酶(LPL)和脂肪酸合成酶(FAS)的活性和mRNA表达水平。将Broodstock喂给四种实验饮食中的一个185天:FO(100%鱼油),FSO(70%鱼油+ 30%豆油),SFO(30%鱼油+ 70%大豆油)左右(100%豆油)。结果表明,肝脏LPL和FAS和卵巢FAS活性和mRNA表达水平相对于预粒细胞的vitellogis和后生成显着增加,除了肝脏LPL mRNA表达外,这两个阶段之间没有显着差异。膳食N-3 LC-PUFA减少组织FAS和增加的LPL活性和mRNA表达水平。卵巢浓度为20:4N-6(ARA),20:5N-3(EPA),22:6N-3(DHA)和N-3 LC-PUFA直接受N-3 LC-PUFA水平的影响。 SO总量的N-3 LC-PUFA浓度低于FO中的57%,而18:2N-6浓度的浓度高4.7 x高于FO。这些结果表明,通过上调LPL和下调Fas,高膳食N-3 LC-PUFA水平在vetellogis期间在vitellogis期间的雌性银POMFRet窝囊中的组织脂质代谢显着影响了组织脂质代谢。

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