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首页> 外文期刊>Applied Soil Ecology >Grazing does not increase soil antibiotic resistome in two types of grasslands in Inner Mongolia, China
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Grazing does not increase soil antibiotic resistome in two types of grasslands in Inner Mongolia, China

机译:放牧在中国内蒙古两种草原中不会增加土壤抗生素抗性

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摘要

Long-term livestock grazing often comes with inputs of manure and urine of animals to grassland soils and can influence the microbial communities. However, the effects of grazing on the diversity and abundance of soil antibiotic resistance genes (ARGs) are still obscure. Here we profiled soil resistome using high-throughput quantitative PCR analysis from desert and typical grasslands (Siziwang and Xilingol steppe, respectively) in Inner Mongolia, each with four grazing intensities (no grazing, light grazing, moderate grazing, and heavy grazing). Totally, 54 and 39 ARGs were detected in the Siziwang steppe and the Xilingol steppe, respectively. The relative abundance of ARGs significantly decreased in the Siziwang steppe or remained unchanged in the Xilingol steppe along the gradient of increasing grazing intensity. Mobile genetic elements (MGEs) were significantly and positively correlated with ARGs in the Siziwang steppe but not in the Xilingol steppe. Structural equation model revealed that grazing indirectly affected ARGs through its impact on MGEs, bacterial diversity, and bacterial community composition. These findings suggest that livestock grazing can impact soil resistome via regulating microbiome in grassland soils. However, grazing intensity has not resulted in a significant impact on the soil resistomes in these desert and typical grasslands.
机译:长期牲畜放牧常常随着动物的粪便和尿液的输入到草地土壤中,可以影响微生物社区。然而,放牧对土壤抗生素抗性基因(Args)的多样性和丰度的影响仍然模糊不清。在这里,我们在内蒙古的沙漠和典型草地(Siziwang和西泠印道狗)使用高通量定量PCR分析进行了土壤抵抗力,每个都有四个放牧强度(没有放牧,轻松放牧,适度放牧和沉重的放牧)。在Siziwang Steppe和西林斯诺斯干草原中检测到完全,54和39次args。沿着增加放牧强度的梯度,在西平王草原中,在西泠印社,在西平醇干草原中,args的相对丰度显着降低。流动遗传元素(MGE)显着且与西泠印社的args显着相关。结构方程模型揭示了通过其对升降,细菌多样性和细菌群落组成的影响进行间接受影响的arg。这些研究结果表明,牲畜放牧可以通过调节草地土壤中的微生物组来撞击土壤抵抗力。然而,放牧强度并未导致这些沙漠和典型草原的土壤电阻的显着影响。

著录项

  • 来源
    《Applied Soil Ecology》 |2020年第1期|共9页
  • 作者单位

    Chinese Acad Sci Res Ctr Ecoenvironm Sci State Key Lab Urban &

    Reg Ecol Beijing 100085 Peoples R China;

    Chinese Acad Sci Res Ctr Ecoenvironm Sci State Key Lab Urban &

    Reg Ecol Beijing 100085 Peoples R China;

    China Agr Univ Coll Land Sci &

    Technol Beijing 100193 Peoples R China;

    Univ Chinese Acad Sci Beijing 100049 Peoples R China;

    Shandong Agr Univ Coll Resources &

    Environm Sci Natl Engn Lab Efficient Utilizat Soil &

    Fertilize Tai An 271018 Shandong Peoples R China;

    Zhejiang Univ Inst Soil &

    Water Resources &

    Environm Sci Zhejiang Prov Key Lab Agr Resources &

    Environm Hangzhou 310058 Peoples R China;

    Inner Mongolia Agr Univ Dept Grassland Sci Coll Ecol &

    Environm Sci Hohhot 010018 Peoples R China;

    Inner Mongolia Agr Univ Dept Grassland Sci Coll Ecol &

    Environm Sci Hohhot 010018 Peoples R China;

    Chinese Acad Sci Res Ctr Ecoenvironm Sci State Key Lab Urban &

    Reg Ecol Beijing 100085 Peoples R China;

    Univ Chinese Acad Sci Beijing 100049 Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 土壤生态学;
  • 关键词

    Grassland soil; Grazing intensity; Antibiotic resistance gene; Mobile genetic elements; Bacterial community;

    机译:草原土壤;放牧强度;抗生素抗性基因;流动遗传元素;细菌群落;

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