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首页> 外文期刊>Applied Soil Ecology >Soil prokaryotes are associated with decreasing Fusarium oxysporum density during anaerobic soil disinfestation in the tomato field
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Soil prokaryotes are associated with decreasing Fusarium oxysporum density during anaerobic soil disinfestation in the tomato field

机译：土壤原核生物与在番茄野野生野生土壤灭菌期间减少镰刀菌密度降低有关

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摘要

Anaerobic soil disinfestation (ASD) is a chemical-independent method that can reduce pathogens, such as Fusarium oxysporum. Although soil microbes play essential roles in ASD, the relationship between the microbial community structure and disinfestation efficiency remains unclear. Thus for improving our understanding of this relationship, we investigated the changes in the microbial community and pathogen density during an ASD period for 14 days in a greenhouse using three different substrates, as wheat bran, sugar-contained diatoms, and dried molasses. Soil samples were collected at 0, 3, 7, and 14 days after ASD treatment. The pathogen densities were analyzed by real-time polymerase chain reactions. Furthermore, prokaryotic community analysis was conducted by using an Illumina Miseq sequencer, and the factors related to pathogen density were statistically analyzed. The pathogen density rapidly decreased by 90% at 3 days after treatment and then slowly decreased until day 14; however the rate of decrease differed among the substrates. On the other hand, the microbial communities were altered after 3 days but recovered to their original state on day 14. The iron reduction level, microbial diversity, richness, and community structure did not correlate with pathogen density. The operational taxonomic units that drastically negatively correlated with pathogen density were Clostridia and Bacilli, both belonging to Firmicutes. This study showed that the changes in the prokaryotic community as a whole did not correlate to ASD efficiency, whereas changes in the abundance of specific microbes in the community were likely related to disinfestation efficiency.
机译：厌氧土壤消毒（ASD）是一种无关的方法，可以减少病原体，例如镰刀菌。虽然土壤微生物在ASD中发挥基本作用，但微生物群落结构与消毒效率之间的关系仍不清楚。因此，为了改善对这种关系的理解，我们在使用三种不同的基材的温室中调查了在ASD期间的微生物群落和病原体密度的变化，用三种不同的基材，作为小麦麸，糖硅氧和干糖蜜。在ASD处理后的0,3,7和14天收集土壤样品。通过实时聚合酶链反应分析病原体密度。此外，通过使用Illumina miseq测序仪进行原核群落分析，统计分析了与病原体密度相关的因素。治疗后3天，病原体密度快速下降了90％，然后慢慢降低至第14天;然而，底物之间的降低速率不同。另一方面，微生物群落在3天后被改变但第14天恢复到原始状态。铁还原水平，微生物多样性，丰富性和群落结构与病原体密度无关。与病原体密度大大与病原体密度相关的操作分类单位是梭菌和Bacilli，无论是属于更强大的。本研究表明，整个原核群落的变化与ASD效率无关，而社区中特定微生物丰富的变化可能与消毒效率有关。

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