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Fertilization strategies affect soil properties and abundance of N-cycling functional genes in an acidic agricultural soil

机译:施肥策略影响酸性农业土壤中的土壤性质和丰度态循环功能基因

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The nitrogen (N) cycle process in agricultural soil is affected by fertilization. However, there is limited information on the abundance of N-cycling microbial community and its response to the different fertilization strategies in acidic soil. Using quantitative PCR, we investigated the microbial abundance involving 11 functional genes of the N cycle (nifH, chiA, aprA, archaeal and bacterial amoA, napA, narG, nirS, nirK, norB and nosZ) in an acidic agricultural soil. The soils were subjected to no fertilizer (Control), chemical NPK fertilizer (NPK), NPK combined with quicklime (NPKCa), NPK combined with crop residues (NPKS), NPK combined with quicklime and crop residues (NPKSCa), NPK combined with manure (NPKM) and only manure (M) fertilization for 27 years. Results showed that fertilization improved crop yield and soil nutrient availability, changed soil pH levels and affected the abundance of N-cycling functional genes. Manure application markedly increased the abundance of all genes and was associated with the largest shift in community structure. The variation of microbial community in the fertilization treatments (NPK, NPKCa, NPKS, NPKSCa, M and NPKM) resulted from the distinct contribution of functional genes, in which the nifH, napA and archaeal amoA genes highly contributed to the community variations between manure fertilization and Control. Soil variables, particularly pH, NO3--N and AP, significantly affected the N-cycling microbial community structure. Overall, the different fertilization strategies demonstrated varying influential patterns on the N-cycling microbial abundance and community by altering soil properties and affecting specific functional gene abundance; in particular, manure treatments had a greater influence compared to chemical fertilizers.
机译:农业土壤中的氮气(n)循环过程受精菌的影响。然而,有关N循环微生物群落丰度的有限信息及其对酸性土壤中不同施肥策略的反应。使用定量PCR,我们研究了酸性农业土壤中的氮素循环(NiFH,Chia,APRA,抗原和细菌AmoA,NoRK,NoSz)的11个功能基因的微生物丰度。对土壤进行肥料(对照),化学NPK肥(NPK),NPK结合Quicklime(NPKCA),NPK与作物残留物(NPKS)结合使用,NPK结合Quicklime和作物残留物(NPKSCA),NPK结合粪便(NPKM),只有粪肥(M)施肥27年。结果表明,施肥改善了作物产量和土壤养分可用性,改变了土壤pH水平并影响了正循环功能基因的丰度。粪便申请明显增加了所有基因的丰富,并且与社区结构的最大转变有关。微生物群落在施肥处理中的微生物群落(NPK,NPKCA,NPK,NPKSCA,M和NPKM)导致功能基因的明显贡献,其中NiFh,NaPa和古氨酰胺基因高度导致粪肥受精之间的群落变化和控制。土壤变量,特别是pH,NO3 - N和AP,显着影响N循环微生物群落结构。总体而言,不同的施肥策略通过改变土壤性质并影响特定的功能基因丰度来表现出正循环微生物丰富和社区的不同影响模式;特别是,与化学肥料相比,粪便治疗具有更大的影响。

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