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首页> 外文期刊>Applied Soil Ecology >Organic amendment mitigates the negative impacts of mineral fertilization on bacterial communities in Shajiang black soil
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Organic amendment mitigates the negative impacts of mineral fertilization on bacterial communities in Shajiang black soil

机译:有机修正减轻矿物施肥对陕江黑土细菌社区的负面影响

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Improvement of Shajiang black soil in the Huanghuai plain is crucial for sustainable agricultural production; however, the effects of intensified fertilizer input on microbial communities in this soil type remain uncertain. Here, we conducted a long-term field trial using a wheat-soybean-maize cropping system, which had five treatments: organic material (soybean cake) (OM), mineral fertilizers (NPK), standard nitrogen (N) (half from OM and half from NPK) (MNPK), high N (1.6-fold higher than other treatments, with half from OM and half from NPK) (HMNPK), and unfertilized control. The organic carbon (C) composition of the soil was analyzed by C-13 nuclear magnetic resonance spectroscopy, and bacterial community structure was determined based on 16S rRNA gene sequencing analysis. Addition of NPK resulted in soil acidification, which was effectively alleviated by organic amendment. NPK significantly (P < .05) reduced the diversity and altered the composition of the bacterial community, although community structure was restored in part by combined organic fertilization. Community variation was limited by soil pH, but not by soil organic C content or chemical composition. Mineral fertilization depleted specific bacterial taxa (e.g., Chitinophagaceae and Nitrospiraceae) associated with the decomposition of polysaccharides and N transformation. Overall, our results suggest that organic amendment can mitigate the negative impacts of mineral fertilization on bacterial communities in Shajiang black soil.
机译:黄淮平原陕江黑土的改善对于可持续农业生产至关重要;然而,强化肥料对这种土壤类型微生物群落的影响仍然不确定。在这里,我们使用麦类 - 大豆玉米种植系统进行了长期的现场试验,其中有五种治疗方法:有机材料(大豆饼)(OM),矿物肥料(NPK),标准氮(N)(来自OM的一半)来自NPK的一半)(MNPK),高N(比其他处理高1.6倍,从OM和NPK的一半)(HMNPK)和未受精控制。通过C-13核磁共振光谱分析土壤的有机碳(C)组合物,基于16S rRNA基因测序分析确定细菌群落结构。添加NPK导致土壤酸化,有机修正有效地减轻了。 NPK显着(p <.05)降低了多样性并改变了细菌群落的组成,尽管部分结构部分通过组合有机施肥恢复。群落变异受土壤pH的限制,但不是土壤有机C含量或化学成分。与多糖分解和N转化的分解相关的矿物施肥耗尽特异性细菌分类群(例如,几丁质焦和零质子)。总体而言,我们的结果表明,有机修正案可以减轻矿物施肥对陕江黑土细菌社区的负面影响。

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