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首页> 外文期刊>Biotechnologie, Agronomie Societe et Environnement >Phylogenetic diversity of Moroccan cork oak woodlands fungi
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Phylogenetic diversity of Moroccan cork oak woodlands fungi

机译：摩洛哥软木栎林真菌的系统发生多样性

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摘要

Interspecific variation among 87 sporocarps of fungi belonging to 15 genera and 39 species were evaluated by analyzing the internal transcribed spacer (ITS) of the rDNA region using restriction fragment length polymorphism (RFLP). The ITS region was first amplified by polymerase chain reaction (PCR) with specific primers and then cleaved with different restriction enzymes. Amplification products, which ranged between 500 and 950 base pairs (bp), were obtained for all the isolates analyzed. The degreeof polymorphism observed did not allow proper identification of most of the species. Cleavage of amplified fragments with the restriction enzymes Alu I, EcoR I and Hinfl revealed extensive polymorphism. The fifteen genera and most species presented specific restriction patterns. The only species not identifiable by a specific pattern belonged to the genera Russula (R. decipiens and R. straminea). These species might be considered as closely related species. The Pisolithus sporocarps had two ITS-RFLP types with one dominating. ITS sequencing confirms that the two RFLP types correspond to two distinct species of Pisolithus. Our data show the potential of ITS region PCR-RFLP for the molecular characterization of ectomycorrhizal fungi and their identification and monitoring in artificial inoculation programs.
机译：通过使用限制性片段长度多态性（RFLP）分析rDNA区的内部转录间隔区（ITS），评估了属于15个属和39个物种的87个真菌果皮中的种间变异。首先用特异性引物通过聚合酶链反应（PCR）扩增ITS区，然后用不同的限制酶切割。对于所有分析的分离物，均获得了介于500和950个碱基对（bp）之间的扩增产物。观察到的多态性程度不能正确鉴定大多数种类。用限制酶Alu I，EcoR I和HinfI切割扩增的片段显示出广泛的多态性。十五属和大多数物种表现出特定的限制模式。不能通过特定模式识别的唯一物种属于红菇属（R. decipiens和R. straminea）。这些物种可能被认为是密切相关的物种。 Pisolithus子果皮有两种ITS-RFLP类型，其中一种占主导地位。 ITS测序证实这两种RFLP类型对应于两种不同的Pisolithus。我们的数据显示了ITS区PCR-RFLP在外生菌根真菌的分子表征以及在人工接种程序中进行鉴定和监测的潜力。

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