...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied biochemistry and biotechnology, Part A. enzyme engineering and biotechnology >Numerical Simulation of Mass Transfer and Three-Dimensional Fabrication of Tissue-Engineered Cartilages Based on Chitosan/Gelatin Hybrid Hydrogel Scaffold in a Rotating Bioreactor
【24h】

Numerical Simulation of Mass Transfer and Three-Dimensional Fabrication of Tissue-Engineered Cartilages Based on Chitosan/Gelatin Hybrid Hydrogel Scaffold in a Rotating Bioreactor

机译：基于壳聚糖/明胶杂交水凝胶支架在旋转生物反应器中的组织工程软骨传质模拟及三维制备

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cartilage tissue engineering is believed to provide effective cartilage repair post-injuries or diseases. Biomedical materials play a key role in achieving successful culture and fabrication of cartilage. The physical properties of a chitosan/gelatin hybrid hydrogel scaffold make it an ideal cartilage biomimetic material. In this study, a chitosan/gelatin hybrid hydrogel was chosen to fabricate a tissue-engineered cartilage in vitro by inoculating human adipose-derived stem cells (ADSCs) at both dynamic and traditional static culture conditions. A bioreactor that provides a dynamic culture condition has received greater applications in tissue engineering due to its optimal mass transfer efficiency and its ability to simulate an equivalent physical environment compared to human body. In this study, prior to cell-scaffold fabrication experiment, mathematical simulations were confirmed with a mass transfer of glucose and TGF-beta 2 both in rotating wall vessel bioreactor (RWVB) and static culture conditions in early stage of culture via computational fluid dynamic (CFD) method. To further investigate the feasibility of the mass transfer efficiency of the bioreactor, this RWVB was adopted to fabricate three-dimensional cell-hydrogel cartilage constructs in a dynamic environment. The results showed that the mass transfer efficiency of RWVB was faster in achieving a final equilibrium compared to culture in static culture conditions. ADSCs culturing in RWVB expanded three times more compared to that in static condition over 10 days. Induced cell cultivation in a dynamic RWVB showed extensive expression of extracellular matrix, while the cell distribution was found much more uniformly distributing with full infiltration of extracellular matrix inside the porous scaffold. The increased mass transfer efficiency of glucose and TGF-beta 2 from RWVB promoted cellular proliferation and chondrogenic differentiation of ADSCs inside chitosan/gelatin hybrid hydrogel scaffolds. The im
机译：据信软骨组织工程提供有效的软骨修复后损伤或疾病。生物医学材料在实现成功的文化和软骨制造方面发挥着关键作用。壳聚糖/明胶杂交水凝胶支架的物理性质使其成为理想的软骨仿真材料。在该研究中，选择一种壳聚糖/明胶杂交水凝胶通过在动态和传统的静态培养条件下接种人脂肪衍生的干细胞（ADSCs）来制造体外的组织工程化软骨。由于其最佳质量传递效率和其模拟与人体相比，模拟等效物理环境的能力，提供动态培养条件的生物反应器在组织工程中得到了更大的应用。在本研究中，在细胞 - 支架制造实验之前，通过旋转壁容器生物反应器（RWVB）和静态培养条件，通过计算流体动力学在旋转壁容器生物反应器（RWVB）和静态培养条件下，确认数学模拟。 CFD）方法。为了进一步研究生物反应器的传质效率的可行性，采用该RWVB在动态环境中制造三维细胞 - 水凝胶软骨构建体。结果表明，与静态培养条件中的培养相比，RWVB的传质效率更快地实现了最终均衡。与静态条件相比，在RWVB中培养的ADSCs在RWVB中培养了三倍。动态RWVB中的诱导细胞培养显示出细胞外基质的广泛表达，而细胞分布在多孔支架内的全细胞外基质充分浸润更均匀地分布。来自RWVB的葡萄糖和TGF-β2的传质效率提高了壳聚糖/明胶杂交水凝胶支架中ADSCs的细胞增殖和软弱化分化。我

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号