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首页> 外文期刊>Applied biochemistry and biotechnology, Part A. enzyme engineering and biotechnology >Detoxification of Organosolv-Pretreated Pine Prehydrolysates with Anion Resin and Cysteine for Butanol Fermentation
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Detoxification of Organosolv-Pretreated Pine Prehydrolysates with Anion Resin and Cysteine for Butanol Fermentation

机译：用阴离子树脂和丁醇发酵的阴离子树脂和半胱氨酸解毒有机溶胶 - 预处理的松氨酸氨酸酯

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摘要

Bioconversion of lignocellulose to biofuels suffers from the degradation compounds formed during pretreatment and acid hydrolysis. In order to achieve an efficient biomass to biofuel conversion, detoxification is often required before enzymatic hydrolysis and microbial fermentation. Prehydrolysates from ethanol organosolv-pretreated pine wood were used as substrates in butanol fermentation in this study. Six detoxification approaches were studied and compared, including overliming, anion exchange resin, nonionic resin, laccase, activated carbon, and cysteine. It was observed that detoxification by anion exchange resin was the most effective method. The final butanol yield after anion exchange resin treatment was comparable to the control group, but the fermentation was delayed for 72 h. The addition of Ca(OH)(2) was found to alleviate this delay and improve the fermentation efficiency. The combination of Ca(OH)(2) and anion exchange resin resulted in completion of fermentation within 72 h and acetone-butanol-ethanol (ABE) production of 11.11 g/L, corresponding to a yield of 0.21 g/g sugar. The cysteine detoxification also resulted in good detoxification performance, but promoted fermentation towards acid production (8.90 g/L). The effect of salt on ABE fermentation was assessed and the possible role of Ca(OH)(2) was to remove the salts in the prehydrolysates by precipitation.
机译：木质纤维素与生物燃料的生物转化患有在预处理和酸水解期间形成的降解化合物。为了实现生物燃料转化的有效生物质，在酶水解和微生物发酵之前通常需要排毒。乙醇有机溶胶预处理的松木的预氢化物用作本研究中丁醇发酵中的底物。研究并进行了六种解毒方法，包括叠加，阴离子交换树脂，非离子树脂，漆酶，活性炭和半胱氨酸。观察到阴离子交换树脂的排毒是最有效的方法。阴离子交换树脂处理后的最终丁醇产率与对照组相当，但发酵延迟72小时。发现添加Ca（OH）（2）以缓解这种延迟并提高发酵效率。 Ca（OH）（2）和阴离子交换树脂的组合导致在72小时内完成发酵和丙酮 - 丁醇 - 乙醇（ABE）的11.11g / L的产生，相当于产率为0.21g / g糖。半胱氨酸排毒也导致良好的排毒性能，但促进了酸生产的发酵（8.90克/升）。评估盐对ABE发酵的影响，Ca（OH）（2）的可能作用是通过沉淀去除静脉内甲酸盐中的盐。

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