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首页> 外文期刊>Applied Organometallic Chemistry >Preparation and synthesis a new chemotherapeutic drug of silver nanoparticle-chitosan composite; Chemical characterization and analysis of their antioxidant, cytotoxicity, and anti-acute myeloid leukemia effects in comparison to Daunorubicin in a leukemic mouse model
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Preparation and synthesis a new chemotherapeutic drug of silver nanoparticle-chitosan composite; Chemical characterization and analysis of their antioxidant, cytotoxicity, and anti-acute myeloid leukemia effects in comparison to Daunorubicin in a leukemic mouse model

机译：制备和合成银纳米粒子 - 壳聚糖复合材料的新型化学治疗药物; 其抗氧化剂，细胞毒性和抗急性髓性白血病的化学特征及分析与白血病小鼠模型中的Daunorubi蛋白相比

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Nanoparticles usually have better outcomes than the bulk samples of the same element because they possess a higher specificity level than the larger particles. This is also true for silver nanoparticles, and little amount of these materials has high remedial effects. Silver nanoparticles are used as a therapeutic tool for the treatment of several diseases such as cancer. In this study, silver nanoparticles using chitosan (AgNPs-chitosan composite) are reported for the first time to exert a dietary therapeutic potential compared to Daunorubicin in an animal model of acute myeloid leukemia. The synthesized AgNPs-chitosan composite was characterized using different techniques including ultraviolet-visible spectroscopy, fourier-transform infrared spectroscopy, energy dispersive X-ray spectrometry, scanning electron microscopy, and transmission electron microscopy. FTIR findings suggested antioxidant compounds in the nanoparticles were the sources of reducing power, reducing silver ions to AgNPs. SEM and TEM images exhibited a uniform spherical morphology and average diameters of 30 nm for the nanoparticles. Then, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging test was done to evaluate the antioxidant potentials of Daunorubicin, AgNO3, chitosan, and AgNPs-chitosan composite. DPPH test revealed similar antioxidant potentials for Daunorubicin and AgNPs-chitosan composite. For the analyzing of cytotoxicity effects of Daunorubicin, AgNO3, chitosan, and AgNPs, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor (MTT) assay was used on HUVEC, 32D-FLT3-ITD, and Murine C1498 cell lines. AgNPs-chitosan composite similar to Daunorubicin had low cell viability dose-dependently against 32D-FLT3-ITD and Murine C1498 cell lines without any cytotoxicity on HUVEC cell line. In vivo design, induction of acute myeloid leukemia was done by 7,12-Dimethylbenz[a]anthracene in 50 mice. Then, the animals were randomly divided into six subgroups, including control, untreated, Daunorubicin, AgNO3, chitosan, and AgNPs-chitosan composite. Similar to Daunorubicin, AgNPs-chitosan composite significantly (P <= .01) decreased the weight of the body, the pro-inflammatory cytokines, and the total white blood cells, blast, neutrophil, monocyte, eosinophil, and basophil counts and increased the anti-inflammatory cytokines and the lymphocyte, platelet, and red blood cell parameters as compared to the untreated mice. These results show that the inclusion of chitosan improves the therapeutic properties of AgNPs-chitosan composite, which led to a significant enhancement in the antioxidant, cytotoxicity, and anti-acute myeloid leukemia activities of the nanoparticles. It appears that AgNPs-chitosan composite can be used as a chemotherapeutic drug for the treatment of acute myeloid leukemia in the clinical trial.
机译：纳米颗粒通常具有比同一元素的散装样品更好的结果，因为它们具有比较大颗粒更高的特异性水平。对于银纳米颗粒，这也是如此，并且很少的这些材料具有很高的补救效果。银纳米粒子用作治疗诸如癌症的几种疾病的治疗工具。在该研究中，首次报告使用壳聚糖（AgNPS-Chotosan复合物）的银纳米颗粒，与急性髓性白血病动物模型中的DaUnorubicin相比，第一次报告饮食治疗潜力。合成的AgNPS-壳聚糖复合材料用不同的技术表征，包括紫外线可见光谱，傅里叶变换红外光谱，能量分散X射线光谱，扫描电子显微镜和透射电子显微镜。 FTIR结果表明纳米颗粒中的抗氧化剂化合物是减少功率的来源，将银离子还原为agnps。 SEM和TEM图像显示纳米颗粒的均匀球形形态和平均直径为30nm。然后，进行1,1-二苯基-2-富铬酰基（DPPH）自由基清除试验，评价Daunorubicin，AgnO3，壳聚糖和AgNPS-壳聚糖复合材料的抗氧化潜力。 DPPH测试显示了Daunorubi蛋白和AgNPS-Chotosan复合材料的相似抗氧化势。用于分析Daunorubicin，AgnO3，壳聚糖和AgNP，3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四溴铵的溴化物（MTT）测定对HUVEC，32D-FLT3-ITD使用和鼠C1498细胞系。类似于Daunorubicin的AgNPS-Chitosan复合材料具有低细胞活力剂，依赖于32d-FLT3-ITD和鼠C1498细胞系，没有任何细胞毒性在Huvec细胞系上。在体内设计中，急性髓性白血病的诱导在50只小鼠中通过7,12-二甲基Benz [A]蒽进行。然后，将动物随机分为六个亚组，包括对照，未处理的，大生霉素，AgNO3，壳聚糖和AgNPS-壳聚糖复合材料。类似于Daunorubi蛋白，AgNPS-Chitosan复合材料显着（p <= .01）降低了体内的重量，促炎细胞因子和全白细胞的总血细胞，爆炸，中性粒细胞，单核细胞，嗜酸性粒细胞和嗜碱性粒细胞计数和增加与未经处理的小鼠相比，抗炎细胞因子和淋巴细胞，血小板和红细胞参数。这些结果表明，壳聚糖的包容性改善了AgNPS-壳聚糖复合材料的治疗性质，其导致纳米粒子的抗氧化剂，细胞毒性和抗急性髓性白血病活性的显着增强。似乎AgNPS-壳聚糖复合材料可用作治疗临床试验中急性髓性白血病的化学治疗药物。

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