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首页> 外文期刊>Aquatic Botany >Identification and characterization of sex-specific markers in the milky mangrove Excoecaria agallocha using double digest restriction site-associated DNA sequencing
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Identification and characterization of sex-specific markers in the milky mangrove Excoecaria agallocha using double digest restriction site-associated DNA sequencing

机译：使用双消化限制性位点相关的DNA测序鉴定和表征乳白色红树鸡excocha agallocha中的性别特异性标记

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摘要

Sex-specific markers can be used to infer sex determination systems of dioecious plants and to study the evolution of plant sex determination. Meanwhile, sex identification of plants with agricultural or economic value using sex-specific markers can be beneficial for rapid breeding practice and better commercial harvest. Although there are more than 15,000 dioecious flowering plants, the sex determination systems of less than 40 species have been identified. A major barrier to studying plant sex determination and evolution is the lack of information on the types of sex determination mechanisms. In this study, we used double digest restriction site associated DNA sequencing (ddRAD-seq) to identify sex-specific markers in a dioecious mangrove tree, Excoecaria agallocha. We performed ddRAD-seq for multiple females and males of E. agallocha in a natural population and identified six candidate male-specific loci among 177,071 catalogue loci for these samples. In contrast, no female-specific loci were found among these catalogue loci. We further validated two of the six male-specific loci using PCR on the individuals used for ddRAD-seq and additional individuals from another geographically distant population. Our results indicated that E. agallocha has a genetic sex determination system. We found that sex ratios in the seeds and one-month-old seedlings of E. agallocha didn't deviate from 1:1 by using PCR amplification of one male specific locus, suggesting post-young-seedling selection may cause skewed sex ratio towards adult males. Reliable sex identification of seedlings of E. agallocha with male-specific markers is valuable for rapid recovery of mangrove forests. The ddRAD method developed here can also be used to identify sex-specific markers in other dioecious plants, especially for trees in which experimental crosses are impractical.
机译：性别特异性标记可用于推断雌雄植物的性测定系统，并研究植物性别测定的演变。同时，使用性别特异性标志物的农业或经济价值的植物的性识别可能是有利于快速育种实践和更好的商业收获。虽然有超过15,000个脱衣性的开花植物，但已经确定了少于40种的性别测定系统。研究植物性别决定和演化的主要障碍是缺乏关于性别决定机制类型的信息。在这项研究中，我们使用了双消化限制性位点相关的DNA测序（DDRAD-SEQ）来识别Diocious红树丛树中的性特异性标记，Excocaria agallocha。我们在自然群体中对大量女性和伊替尔科的疟原虫进行了DDRAD-SEQ，并确定了六个候选男性特定的基因座，在177,071目录基因座中进行这些样品。相比之下，这些目录基因座中没有发现特定于女性的基因座。我们进一步验证了使用用于DDRAD-SEQ和其他地理位置遥远人口的额外个体的个人PCR的六个男性特异性基因座中的两个。我们的结果表明，E.Agallocha具有遗传性别测定系统。我们发现，通过使用PCR扩增一个男性特异性基因座的种子和0个月龄幼苗的性别比率并未偏离1：1，表明年轻幼苗选择可能导致偏斜性比例成年男性。具有男性特异性标记的E. agallocha的可靠性别鉴定agallocha对红树林森林的快速恢复有价值。这里开发的DDRAD方法也可用于识别其他脱衣植物中的性特异性标记，特别是对于实验交叉是不切实际的树木。

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