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首页> 外文期刊>Archives of Animal Nutrition >Effect of dietary nitrogen level and source on mRNA expression of urea transporters in the rumen epithelium of fattening bulls
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Effect of dietary nitrogen level and source on mRNA expression of urea transporters in the rumen epithelium of fattening bulls

机译：膳食氮素水平与源对肥胖牛头瘤瘤上皮尿素转运蛋白MRNA表达的影响

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This paper aims to study the effect of the dietary treatments on mRNA expression of urea transporter B (UT-B) and some aquaporins (AQP) in rumen epithelium of Italian Simmental young bulls. Eighty animals allocated to 16 pens were fed from about 500 to 650kg body weight with four experimental diets, which resulted from the combination of two crude protein levels (125 and 110g/kg dry matter, diets M and L, respectively) and two nitrogen sources (soybean meal (SBM) or SBM partly replaced by an isonitrogenous mixture of corn and urea; diets -U and +U, respectively). At slaughtering samples of blood and rumen epithelium were collected from six bulls for each diet. Blood samples were analysed for haematological parameters and quantitative PCR was carried out on the mRNA extracted from the rumen epithelium samples. The bulls fed diets M had lower plasma concentrations of aspartate aminotransferase than those receiving diets L (78.9 vs. 88.3U/l, p=0.04). Plasma urea was higher (p=0.03) for diets M and lower for diets +U (2.0 vs. 2.5 and 1.73 vs. 2.00mmol/l, respectively, in M and L diets, p=0.04). The effect of dietary treatments on rumen UT expression were limited to AQP3, which was down regulated (p=0.01) in diets +U. Finally, a high positive correlation (R-2=0.871) between the expressions of AQP7 and AQP10 was found. In conclusion, the AQP3 appears very responsive to dietary treatments and therefore it is a candidate to be further studied in rumen metabolism experiments. The close relationship between mRNA expression of AQP7 and AQP10 indicates a similar function of these two proteins.
机译：本文旨在研究饮食治疗对意大利半公牛瘤胃上皮瘤胃上皮细胞膜上皮细胞的MRNA表达的影响。将八十只动物分配为16张，用四种实验饮食从约500至650kg体重喂食，这是由两种粗蛋白水平（125和110g / kg干物质，饮食M和L）和两个氮源的组合产生的（大豆饭（SBM）或SBM部分被玉米和尿素混合物替换;饮食-u和+ U）。在屠宰血液和瘤胃上皮样品，从六公牛队收集每种饮食。分析血液样品进行血液学参数，并在从瘤胃上皮样品中提取的mRNA上进行定量PCR。喂食饮食M的公牛患者血浆浓度降低了天冬氨酸氨基转移酶的血浆浓度，而不是接受饮食L（78.9与88.3U / L，P = 0.04）。饮食M和饮食+ u（2.0 vs.2.5和1.73分别为2.00mol / L，在M和L饮食中，血浆尿素较高（p = 0.03），+ u（2.0 vs.2.5和1.73，P = 0.04）。饮食治疗对瘤胃的影响限于AQP3，其在饮食+ U中被调节（p = 0.01）。最后，发现AQP7和AQP10的表达之间的高正相关（R-2 = 0.871）。总之，AQP3对膳食处理非常敏感，因此在瘤胃代谢实验中进一步研究了候选者。 AQP7和AQP10 mRNA表达的密切关系表明这两种蛋白质的类似功能。

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