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Modelling of sulphur amino acid requirements and nitrogen endogenous losses in kittens

机译：硫氨基酸要求和小猫氮素内源性损失的建模

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摘要

The objective of this study was to estimate the sulphur amino acid (methionine + cystine) requirements and nitrogen endogenous losses in kittens aged 150 to 240 d. Thirty-six cats were distributed in six treatments (six cats per treatment) consisting of different concentrations of methionine + cystine (M + C): T1, 6.5 g/kg; T2, 8.8 g/kg; T3, 11.3 g/kg; T4, 13.6 g/kg; T5, 16.0 g/kg; and control, 6.5 g/kg. Diets were formulated by serial dilution of T5 (a diet relatively deficient in M + C but containing high protein concentrations) with a minimal nitrogen diet (MND). Thus, crude protein and amino acid concentrations in diets T1-T5 decreased by the same factor. The control diet was the T1 diet supplemented with adequate concentrations of M + C (6.5 g/kg; 8.8 g/kg; 11.3 g/kg; 13.6 g/kg and 16.0 g/kg). All diets were based on ingredients commonly used in extruded cat diets. Digestibility assays were performed for the determination of nitrogen balance. Nitrogen intake (NI) and nitrogen excretion (NEX) results data were fitted with an exponential equation to estimate nitrogen maintenance requirement (NMR), theoretical maximum for daily nitrogen retention (NRmaxT), and protein quality (b). M + C requirements were calculated from the limiting amino acid intake (LAAI) equation assuming a nitrogen retention of 45 to 65% NRmaxT. The NMR of kittens aged 150, 195, and 240 d was estimated at 595, 559, and 455 mg/kg body weight (BW)(0.67)per day, respectively, and M + C requirements were estimated at 517, 664, and 301 mg/kg BW(0.67)per day, respectively.
机译：本研究的目的是估计硫氨基酸（甲硫氨酸+胱氨酸）要求和氮素内源性损失在150至240℃。三十六只猫分布于六种治疗方法（每次治疗六只猫），由不同浓度的蛋氨酸+胱氨酸（M + C）：T1,6.5g / kg;; T2，8.8克/千克; T3，11.3克/千克; T4,13.6 g / kg; T5,16.0 g / kg;并控制，6.5克/千克。通过连续稀释T5（饮食相对缺乏M + C但含有高蛋白质浓度的饮食）的饮食配制饮食，具有最小的氮气饮食（MND）。因此，粗蛋白质和饮食中的氨基酸浓度T1-T5的含量减少相同的因素。对照饮食是补充的T1饮食，其具有足够的M + C（6.5g / kg; 8.8g / kg; 11.3g / kg; 13.6g / kg和16.0g / kg）。所有饮食基于常用于挤压猫食的成分。进行消化测定以确定氮气平衡。氮气进气（Ni）和氮排泄（NEX）结果数据具有指数方程，以估计氮维护要求（NMR），每日氮保留（NRMAXT）和蛋白质质量（B）的理论最大值。假设氮保留为45至65％NRMAXT，从限制氨基酸摄入（LAAI）方程计算M + C要求。估计在595,559和240d的kittens的核磁共振估计每天595,559和455mg / kg体重（bw）（0.67），估计M + C要求在517,664和每天301毫克/千克BW（0.67）。

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