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首页> 外文期刊>Archives of microbiology >Detection of a phage genome carrying a zonula occludens like toxin gene (zot) in clinical isolates of Stenotrophomonas maltophilia
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Detection of a phage genome carrying a zonula occludens like toxin gene (zot) in clinical isolates of Stenotrophomonas maltophilia

机译：检测患有毒素基因（ZOT）的噬菌体基因组在Stenotophomonas麦芽蛋白的临床分离株中的噬菌体基因组

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摘要

During a study of the genetic diversity of Stenotrophomonas strains, we found an autonomous replicating DNA molecule in chromosomal DNA preparations of the clinical Stenotrophomonas maltophilia strain c5. The entire sequence of 6,907 bp of the isolated DNA molecule was determined, which was called phi SMA9. Seven ORFs, which code for proteins with considerable similarity to proteins in databases, were identified in the DNA sequence. The largest ORF shows high sequence similarities to the pI protein of the filamentous phage phi Lf, which was later shown to be identical to toxin Zot of Vibrio cholerae. Beside the Zot-like protein, six other proteins with similarities to known phage proteins such as a phage replication protein RstA and phage absorption or coat protein are encoded on phi SMA9, which indicate that this circular DNA molecule represents the replicative form of a linear phage genome. A PCR-based screening showed that only five from the totally investigated 47 Stenotrophomonas strains of clinical and environmental origin harbor these genes. Altogether, we describe the first genome of a phage for the nosocomial pathogen Stenotrophomonas, which contains a Zot toxin like gene and might be regarded as the first Stenotrophomonas virulence factor.
机译：在研究Stenotophomonas菌株的遗传多样性期间，我们发现一种在临床牙科疗法菌株C5的染色体DNA制剂中的自主复制DNA分子。测定分离的DNA分子的6,907bp的整个序列，称为phi sma9。七ORF，在DNA序列中鉴定了与数据库中具有相当相当相似性的蛋白质的代码。最大的ORF显示与丝状噬菌体PHI LF的PI蛋白的高序列相似性，后来显示与霍乱霍乱的毒素Zot相同。除了像素样蛋白质旁边，在PHI SMA9上编码六种具有与已知噬菌体蛋白质的相似性的其他蛋白质，这表明该圆形DNA分子代表了线性噬菌体的复制形式基因组。基于PCR的筛选表明，只有五个来自全面研究的47个狭窄的临床和环境来源的肠道菌株留下这些基因。总共合，我们描述了噬菌体病原体牙科孢子酵母的噬菌体的第一个基因组，其含有毒素，如基因，并且可能被认为是第一牙科霉菌毒力因子。

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